Overview
Ester fluorescent probes are specialized chemical tools designed to detect and quantify esterase activity through fluorescence emission. These compounds contain an ester bond that is cleaved by esterase enzymes, triggering a fluorescent signal. They are widely used in biochemical research, pharmaceutical development, and clinical diagnostics. These probes typically consist of three components: a fluorophore (light-emitting molecule), an ester group (enzyme substrate), and sometimes a quenching group. When the ester bond is hydrolyzed by esterases, the fluorescent signal is either activated or modified, allowing researchers to monitor enzyme activity in real-time.
Physical and Chemical Properties
The physical properties of ester fluorescent probes vary significantly depending on their specific chemical structure. Most are stable at room temperature when properly stored but may degrade under prolonged exposure to light, heat, or moisture. Their molecular weights typically range from 300-800 g/mol. Chemically, these probes are designed to be selectively recognized by esterase enzymes while maintaining fluorescence stability. Many exhibit high quantum yields (brightness) and large Stokes shifts (difference between excitation and emission wavelengths), which are advantageous for biological imaging. Their solubility characteristics are carefully balanced to allow cellular permeability while maintaining sufficient aqueous solubility for biological applications.
Main Applications
In biomedical research, ester fluorescent probes are indispensable for studying enzyme kinetics, measuring esterase activity in cells, and screening potential enzyme inhibitors. They enable non-invasive monitoring of enzymatic processes in living cells and tissues through fluorescence microscopy or flow cytometry. Clinical applications include diagnostic tests for esterase-related disorders and drug metabolism studies. Pharmaceutical companies use these probes in high-throughput screening of drug candidates. Additionally, they find use in environmental monitoring to detect esterase activity in microorganisms and assess pollutant degradation.
Safety and Storage
Proper handling of ester fluorescent probes requires standard laboratory safety precautions. Most probes are considered irritants and should be handled with gloves and eye protection in a fume hood. Some may be light-sensitive and require amber vials or foil wrapping. For long-term storage, most probes should be kept at -20°C in a desiccator to prevent moisture absorption and degradation. Aliquotting is recommended to minimize freeze-thaw cycles. Solutions should be prepared fresh or stored at -80°C for short periods, as many probes are unstable in solution over time.
B2B Procurement Guide
When procuring ester fluorescent probes commercially, verify the certificate of analysis for purity (typically >95%) and confirm the excitation/emission wavelengths match your equipment specifications. Consider the probe's enzymatic specificity (some target specific esterase isoforms) and cellular permeability if used in live-cell imaging. For bulk orders, request custom synthesis documentation and stability data. Leading suppliers often provide technical support for assay optimization. Price varies significantly based on complexity, with custom-designed probes commanding premium prices. Consider lead times for special orders, which may range from 2-8 weeks depending on complexity.
Related Manufacturers
- 主营：cy荧光染料、ICG吲哚菁绿、源头厂家
- 主营：6-二羟基吲哚、二羟基吲哚啉氢溴酸、水解角蛋白、L-丙交酯、4-联苯甲醇丙烯酸酯、乙交酯、D(+)-丙交酯、7-去氢胆固醇、石胆酸钠盐、熊去氧胆酸钠盐、2-辛基十二醇、2-巯基噻唑啉、2-巯基苯并咪唑、苯基周位酸(8-苯胺、半叶素、乙烯利、环丙磺酰胺、苄氧基胺盐酸盐、1-氨基环丙烷羧酸、4-羟乙烯基氧基二苯、咪鲜胺、可比落
- 主营：胆酸、半叶素、二羟基吲哚、L-丙交酯、缩合剂、生物缓冲液、表面活性剂、硅烷偶联剂、光电材料中间体、精细化工
- 主营：催化剂、peg试剂、cy5四氮、芘丁酸酯、活性脂、苯二胺、2406-34-0、乙氧基、试剂cas、环辛烯、生物素、cb-cyclam、实验室、dbco-acid、半乳糖、溴苯基、交联剂、环己炔、荧光素、3694-51-7、ac4galnal、2001-96-9、葡萄糖、环辛炔、直发cas、糖化学
- 主营：反应活、hydrazide、修饰剂、AMCANHS活化酯、催化剂、糖蛋白、连接子、激动剂、聚合物、活性脂、胆固醇、钆特酸、催化性、dna标记、络合剂、荧光试剂、雨蛙素、乙二胺、ped结构、衍生物、化合物、链接剂、反应物、生物素
- 主营：在水中、包含肽、含叠氮、BAPTA琥珀酰亚胺酯、丙炔基、由氨基、菁染料、peg单元、具有炔、胺hcl盐、阿尔法、催化剂、peg试剂、结构式、氨基nh2、3694-51-7、碘化物、4727-50-8、具有两、带有炔、磺酸基、3065-79-0、aminopeg8、甲基九、bcn基团、荧光素
- 主营：抗氧剂、氧化剂、紫外线吸收剂、荧光增白剂、催化剂、还原剂、缩合剂、光引发剂、酶制剂、成核剂、生物缓冲液、表面活性剂、检测试剂、光电材料中间体、改性剂、指示剂、多肽、染料中间体、固醇系列、酶溶液
- 主营：联吡啶、甘氨酰、丁烯酸、异丁酯、阻燃剂、酸性橙、西瓜酮、硫酸锰、氯丁烷、甲醇钙、聚合物、肌氨酸、咯喹酮、烷硫酮、磷酸铵、锑酸钠、双噻吩、磷酸铁、中性红、碳酸镍、磷酸铜、氨丁基、浅铬黄、八(6-溴
- 主营：丙烯酸酯、荧光染料、花青素染料
- 主营：金纳米、三角片、麦芽糖、荧光探针Cy3、羟丙基、ito薄膜、氮化钛、tio2纳米、碳粉tio2、修饰tio2、pd纳米笼、水溶伊红、纳米复合、磁性纳米、蛋白芯片、包硅金银、硅纳米线、包硅纳米、硅纳米管、螯合树脂、油溶性银、复合材料、薄膜定制、纳米粒子
- 主营：染料cy3、ifluor820、50x荧光、双nhs酯、探针hpf、探针apf、ifluor790、试剂盒、ifluor647、1mg蛋白、pcr定量、ifluor860、atp分析、橙钠盐、ifluor460、rna定量、派诺宁、生物素、刚果红、cy7酰肼、蛋白酶、ifluor810、抑制剂、funxite-1、封闭剂
- 主营：检测试剂盒、Elisa试剂盒、农残检测试剂盒、钙离子荧光探针Cal、试剂、液相检测、测试盒、检测卡、比色法检测、荧光法检测
- 主营：活性脂、三苯胺、mof材料、琥珀酰亚胺酯、月桂酸甘油酯、聚乙二醇、海藻酸钠、聚苯乙烯、蛋白交联剂、二元醛基cof单体
- 主营：钙离子荧光探针Cal、试剂盒、抗体、试剂
- 主营：嵌段共聚物、合成磷脂、荧光染料、苯硼酸酯ONOO、PEG、点击化学、量子点