Overview
Horse Stem Cell Factor (SCF) is a cytokine that plays a pivotal role in the survival, proliferation, and differentiation of hematopoietic stem cells. It binds to the c-Kit receptor, activating signaling pathways essential for cell growth. Derived from equine sources, it is widely used in veterinary and biomedical research to study stem cell biology and develop therapies for conditions like anemia and immune disorders. SCF is produced as a recombinant protein, ensuring consistency and purity for laboratory use. Its applications extend to in vitro cell culture systems, where it supports the expansion of stem cells for experimental or therapeutic purposes. The protein is available in lyophilized or liquid forms, with strict storage requirements to maintain stability.
Physical and Chemical Properties
Horse SCF is a glycosylated protein with a molecular weight of approximately 18-20 kDa. It typically appears as a lyophilized powder or in solution, soluble in standard aqueous buffers like PBS or culture media. The protein’s stability depends on proper storage, with recommended temperatures at -20°C for long-term preservation. Its biological activity is measured in units (U) or nanograms (ng), with potency verified through cell proliferation assays. The protein is sensitive to repeated freeze-thaw cycles, which can degrade its efficacy. Suppliers often provide certificates of analysis detailing purity (usually >95%) and endotoxin levels (<1 EU/μg), critical for research reproducibility.
Main Applications
In veterinary medicine, Horse SCF is used to study hematopoietic disorders and develop treatments for conditions like aplastic anemia. It is also employed in regenerative medicine to enhance the growth of stem cells for tissue repair. Researchers utilize it in cell culture systems to maintain or expand stem cell populations for experimental models. Beyond veterinary applications, Horse SCF contributes to comparative studies in human stem cell research, offering insights into cross-species cytokine functions. Its role in immunology includes modulating mast cell development, making it valuable for allergy and inflammation research. The protein’s versatility underscores its importance in both basic and applied sciences.
Safety and Storage
Handling Horse SCF requires standard laboratory precautions, including gloves and protective eyewear to prevent exposure. The protein should be reconstituted in sterile, endotoxin-free buffers to avoid contamination. Storage at -20°C is recommended, with aliquoting to minimize freeze-thaw cycles that could compromise activity. Disposal should follow institutional guidelines for biological materials. Suppliers provide safety data sheets (SDS) outlining hazards, though the protein is generally low-risk when handled properly. Stability studies suggest a shelf life of up to 12 months when stored correctly, though users should verify activity before critical experiments.
B2B Procurement Guide
When purchasing Horse SCF, buyers should prioritize suppliers with proven track records in recombinant protein production. Key criteria include purity (>95%), endotoxin levels (<1 EU/μg), and biological activity certification. Bulk orders may qualify for discounts, but ensure proper storage capacity to maintain product integrity. Procurement teams should request detailed technical data, including batch-specific analyses and storage recommendations. Lead times vary by supplier, so plan orders accordingly. For research institutions, partnering with vendors offering validated protocols and customer support can streamline experimental workflows. Price ranges typically span $200-$500 per mg, depending on quantity and supplier reputation.
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