Overview
Aldehyde dehydrogenase (Ald) is a family of enzymes that catalyze the oxidation of aldehydes to their corresponding carboxylic acids, using NAD(P)+ as a cofactor. These enzymes are found in nearly all organisms and play crucial roles in metabolic pathways, particularly in the detoxification of reactive aldehydes. In humans, Ald is essential for alcohol metabolism, converting toxic acetaldehyde to acetate. The enzyme also participates in other metabolic processes, including amino acid catabolism and the metabolism of neurotransmitters. Genetic variations in Ald genes can lead to conditions like alcohol flush reaction and increased cancer risk.
Physical and Chemical Properties
Ald exists as a homotetramer or homodimer in its active form, with each subunit containing a binding site for NAD(P)+ and the aldehyde substrate. The enzyme operates optimally at physiological pH (7.4-8.0) and temperature (37°C for human isoforms). Different isoforms of Ald show varying substrate specificities and kinetic properties. For instance, mitochondrial Ald2 has high affinity for acetaldehyde, while cytosolic Ald1 prefers aromatic aldehydes. The enzyme's activity can be inhibited by disulfiram (Antabuse), a drug used in alcohol aversion therapy.
Main Applications
In the pharmaceutical industry, Ald is studied for its role in drug metabolism and as a target for medications. Researchers investigate Ald inhibitors for potential treatments of alcohol dependence and certain cancers where aldehyde accumulation occurs. Biotechnology applications include using Ald in biosensors for aldehyde detection and in synthetic biology pathways for chemical production. Industrial processes employ microbial Ald for the production of carboxylic acids from aldehydes in bio-based manufacturing.
Safety and Storage
As a biological material, purified Ald requires careful handling to maintain activity. The enzyme should be stored at -20°C or lower in glycerol-containing buffers to prevent denaturation. Lyophilized forms are more stable but must be reconstituted properly. While not highly toxic, standard laboratory precautions should be followed when working with Ald. This includes wearing appropriate personal protective equipment and working in properly ventilated areas, especially when handling large quantities or working with potential inhibitors.
B2B Procurement Guide
When sourcing Ald for research or industrial use, consider the specific isoform required for your application. Suppliers typically provide Ald from various sources (human, microbial) with different purity levels and activity units. Key specifications to verify include: enzymatic activity (units/mg), purity level (by SDS-PAGE), presence of stabilizing agents, and storage conditions. For large-scale industrial use, consider microbial-produced recombinant Ald for cost-effectiveness. Lead times for custom preparations can range from 2-8 weeks depending on the supplier and product specifications.
Related Manufacturers
- 主营：乙醇胺、mpeg-maca、甲醚酰肼、生物素聚乙二醇丙醛、单甲醚乙酸、单甲醚氨基、马来酰亚胺、单甲醚马来、单甲醚叠氮、单甲醚丙炔、单甲醚卤代烃、单甲醚苯甲醛、甲醚环氧丙烷、甲基丙烯酸酯、甲醚二苯基环、甲醚异氰酸酯、单甲醚乙烯基、聚乙二醇单甲醚
- 主营：elisa试剂盒、生化试剂盒、抗体、细胞、pcr试剂盒
- 主营：催化剂、peg试剂、cy5四氮、生物素、活性脂、苯二胺、2406-34-0、乙氧基、试剂cas、环辛烯、cb-cyclam、实验室、dbco-acid、半乳糖、溴苯基、交联剂、环己炔、荧光素、3694-51-7、ac4galnal、2001-96-9、葡萄糖、环辛炔、直发cas、糖化学
- 主营：生物素PEG丙醛、荧光染料、花青素染料
- 主营：微生物elisa试剂盒、elisa试剂盒
- 主营：elisa试剂盒、检测试剂盒、酶免试剂盒、通蔚生物试剂盒、elisakit
- 主营：试剂盒、酶联免疫检测试剂盒、检测试剂盒、人锚定蛋白、免疫球蛋白、人醛脱氢酶、磷脂酶域蛋白、人乙醇脱氢酶、寡聚化域蛋白、抗突触核蛋白、人层粘连蛋白、易位断点蛋白、人特异性蛋白、病毒样核蛋白、诱导反应蛋白、突触关联蛋白、人动力蛋白胞浆、前列腺素还原酶、人突触囊泡蛋白、溶酶体辅助蛋白、人血管抑制蛋白、磷脂酰肌醇聚糖
- 主营：合成酶、微量法、dna提取、糖蛋白、试剂盒、脂蛋白、人落叶、测试盒、果汁中、dnamarker、人新生、模拟abo、dna抗体、赫澎人、极速pcr、人免疫、病毒igg、人叶酸、双链dna、雄二酮、人睾酮、人多肽、干扰素、食欲素、人血管
- 主营：白细胞介素、甲状腺素、活性氧簇、生物试剂、促甲状腺激素、孕酮、促黄体生成素、心肌肌钙蛋白、检测试剂盒、植物试剂盒、试剂盒、实验室试剂、Elisa、定量分析、定性分析、酶联免疫试剂盒、科研试剂盒、免费代测
- 主营：人ELISA试剂盒、大鼠ELISA试剂盒、生化法检测试剂盒、分子学检测试剂盒、植物ELISA试剂盒、鱼ELISA试剂盒
- 主营：试剂盒、试剂瓶、白吸头、蓝吸头、黄吸头、实验服务、ELISA免费实验代测、Western实验服务、科研试剂盒
- 主营：ELISA试剂盒、ELISA检测试剂盒、小鼠ELISA试剂盒、人ELISA试剂盒、大鼠ELISA试剂盒、试剂盒elisa
- 主营：ELISA试剂盒、小鼠ELISA试剂盒、细胞因子检测试剂盒、人ELISA检测试剂盒、姐检测试剂盒、酶免检测试剂盒、生化试剂盒、酶联免疫吸附测定试剂盒
- 主营：ELISA试剂盒、PCR试剂盒、生化试剂盒、蛋白、抗体、细胞、检测试剂盒
- 主营：反应活、hydrazide、修饰剂、衍生物、生物素、催化剂、糖蛋白、连接子、激动剂、聚合物、活性脂、胆固醇、钆特酸、催化性、dna标记、络合剂、荧光试剂、雨蛙素、乙二胺、ped结构、化合物、链接剂、反应物