Overview
Endoglin is a homodimeric transmembrane glycoprotein predominantly expressed on vascular endothelial cells. First identified in 1992, it serves as an accessory receptor in the TGF-β signaling pathway and is crucial for cardiovascular development and angiogenesis. The protein exists in two isoforms (L-ENG and S-ENG) generated by alternative splicing, with molecular weights of approximately 180 kDa and 160 kDa respectively when glycosylated. In clinical contexts, endoglin is recognized as CD105 in the CD classification system. Its expression is upregulated in activated endothelial cells during tumor angiogenesis, making it a valuable target for anti-cancer therapies and a diagnostic marker for vascular pathologies including hereditary hemorrhagic telangiectasia (HHT).
Physical and Chemical Properties
Endoglin's extracellular domain contains a zona pellucida (ZP) module and an orphan domain that facilitate ligand binding. The protein undergoes N-linked glycosylation at multiple sites, which affects its molecular weight and functional interactions. Its cytoplasmic domain lacks intrinsic kinase activity but mediates signal transduction through interactions with ALK1 and other TGF-β receptors. The recombinant form used in research typically exhibits >90% purity by SDS-PAGE and maintains biological activity in cell-based assays. Stability studies show the protein remains active for ≥12 months when stored at -20°C in lyophilized form. Soluble endoglin (sENG), a proteolytic cleavage product, has been identified as a circulating marker in preeclampsia and other vascular disorders.
Main Applications
In research settings, endoglin antibodies are extensively used for immunohistochemical staining of vascular tissues to study angiogenesis in cancer models. The soluble extracellular domain is employed in ELISA kits for quantifying circulating levels in cardiovascular studies. Pharmaceutical companies target endoglin with monoclonal antibodies (e.g., carotuximab) in clinical trials for solid tumors. Industrial applications include quality control of endothelial cell cultures for tissue engineering and the development of vascular-targeted drug delivery systems. Diagnostic labs utilize endoglin detection in HHT genetic testing and preeclampsia risk assessment panels. Recent studies explore its role in fibrotic diseases and wound healing applications.
Safety and Storage
Laboratory-grade endoglin poses minimal hazard when handled according to standard biosafety level 1 (BSL-1) protocols. Recombinant proteins should be reconstituted in sterile PBS or culture-grade buffers without preservatives that might affect protein folding. Working solutions are typically stable for 1-2 weeks at 4°C. For long-term preservation, lyophilized material should be stored at -20°C in sealed containers with desiccant. Avoid repeated freeze-thaw cycles of liquid aliquots, which can lead to protein aggregation. Contamination risks are mitigated by using endotoxin-free preparations (typically <0.1 EU/μg) for cell culture applications.
B2B Procurement Guide
When sourcing endoglin for industrial or research use, verify the supplier's documentation of biological activity (e.g., binding affinity to TGF-β1/TGF-β3) and species reactivity. For antibody production or diagnostic applications, request certificates analyzing glycosylation patterns that may affect immunogenicity. Bulk purchasers should negotiate batch-to-batch consistency guarantees, with HPLC and mass spectrometry characterization data. Consider suppliers offering GMP-grade material for clinical applications, which undergoes additional viral safety testing. Lead times for custom recombinant variants (e.g., tagged proteins) typically range 8-12 weeks. Some manufacturers provide technical support for assay development and validation.
Related Manufacturers
- 主营：ELISA试剂盒、PCR试剂盒、生化试剂盒、内皮糖蛋白ELISA试剂盒、蛋白、抗体、细胞、检测试剂盒
- 主营：elisa 试剂盒、Elisa试剂盒、进口试剂盒、国产试剂盒、试剂、细胞、ELISA检测试剂盒、酶联免疫法、酶联免疫试剂盒、酶活性检测、酶标仪检测
- 主营：试剂盒、人25羟基维生素、人低氧诱导因子
- 主营：ELISA试剂盒、高灵敏度试剂盒、试剂盒厂家、人ELISA试剂盒、小鼠ELISA试剂盒、大鼠ELISA试剂盒、仓鼠ELISA试剂盒、豚鼠ELISA试剂盒、鸡ELISA试剂盒、鸭ELISA试剂盒、牛ELISA试剂盒、羊ELISA试剂盒、马ELISA试剂盒、猴ELISA试剂盒、猪ELISA试剂盒、犬ELISA试剂盒、兔ELISA试剂盒、鱼ELISA试剂盒、植物ELISA试剂盒、土壤ELISA试剂盒、定性ELISA试剂盒、肿瘤标志物检测ELISA试剂盒、特种蛋白检测ELISA试剂盒、传染病检测ELISA试剂盒
- 主营：科研试剂盒、酶免试剂盒、elisa试剂盒、犬内皮糖蛋白、细胞、生物试剂、进口ELISA试剂盒、PCR试剂盒、生化测试盒
- 主营：白细胞介素、生物试剂、甲状腺素、内皮糖蛋白ELISA试剂盒、活性氧簇、促甲状腺激素、孕酮、促黄体生成素、心肌肌钙蛋白、检测试剂盒、植物试剂盒、试剂盒、实验室试剂、Elisa、定量分析、定性分析、酶联免疫试剂盒、科研试剂盒、免费代测
- 主营：人内皮糖蛋白、试剂盒、信号调节激酶、人转谷氨酰胺酶
- 主营：ELISA试剂盒、生化检测试剂盒、PCR试剂盒、大鼠ELISA试剂盒、小鼠ELISA试剂盒、兔ELISA试剂盒、鸡ELISA试剂盒
- 主营：ELISA试剂盒、PCR试剂盒、生化试剂盒、CD105内皮糖蛋白ELISAKit、脂蛋白、关基因、亲环素、钾通道、列抗体、ntp溶液、蛋白酶、脑蛋白、病毒抗、蛋白质、239抗体、dtt溶液、科研试剂
- 主营：内皮糖蛋白试剂盒、elisa试剂盒、试剂盒、抗体
- 主营：人内皮细胞蛋白C受体、ELISA试剂盒、抗体
- 主营：Elisa试剂盒、生化试剂盒、蛋白试剂盒、小鼠可溶性内皮糖蛋白、细胞试剂盒
- 主营：试剂盒、同源物、脂蛋白、合蛋白、粘液素、抑制素、亲环素、保护素、信号素、胞色素、脂肪酶、末端肽、磷脂酶、蛋白酶、变位酶、抑制剂、脱水酶、抑制物、凝蛋白、激活肽、连接酶、样激酶、解氨酶、恒温箱、利钠肽
- 主营：氯霉素、试剂盒、脂蛋白、糖蛋白、胆固醇、钙蛋白、粘液素、调蛋白、磷酸酶、人嗅素、饥饿素、磷脂酶、抑制因、凝血酶、连接酶、凝集素、分泌酶、白介素、胰岛素、阻滞剂、结合盒、防御素、纤溶酶、干扰素、板层素
- 主营：Elisa试剂盒、人elisa试剂盒、大鼠elisa试剂盒、大鼠可溶性内皮糖蛋白、小鼠elisa试剂盒、植物elisa试剂盒、酶联免疫试剂盒