Overview
Mouse double-stranded RNA (dsRNA) is a laboratory-synthesized molecule designed to mimic the structure of viral RNA. It serves as a critical tool in biomedical research, particularly for studying RNA interference (RNAi) pathways and immune system activation. Unlike single-stranded RNA, dsRNA's complementary strands form a stable duplex structure that is recognized by cellular machinery. Researchers commonly use it to silence specific genes in mouse models or to stimulate antiviral immune responses in experimental systems.
Physical and Chemical Properties
Mouse dsRNA typically appears as a white lyophilized powder or clear solution when dissolved. Its molecular weight depends entirely on the designed sequence length, which commonly ranges from 21-500 base pairs for research applications. The molecule is highly soluble in nuclease-free aqueous buffers but susceptible to degradation by RNases in the environment. Its UV absorbance profile shows a characteristic peak at 260 nm, with an A260/A280 ratio of approximately 2.0 indicating pure RNA.
Main Applications
In molecular biology, mouse dsRNA is primarily used to trigger RNA interference, enabling targeted gene knockdown studies. Researchers employ it to investigate gene function in murine cell lines or animal models. The immunology field utilizes dsRNA to study pattern recognition receptors like TLR3 and MDA5. Pharmaceutical developers use it to model viral infections and test antiviral compounds. Recent applications include vaccine adjuvant research and cancer immunotherapy development.
Safety and Storage
Proper handling requires RNase-free techniques to prevent degradation. Use DEPC-treated water and certified RNase-free tubes for preparation. Gloves should be worn at all times as skin RNases can rapidly degrade the material. For storage, aliquoting is recommended to minimize freeze-thaw cycles. Lyophilized dsRNA remains stable for years at -80°C, while solutions typically maintain integrity for 6-12 months at -20°C. Avoid exposure to high temperatures or repeated pH changes.
B2B Procurement Guide
When sourcing mouse dsRNA, buyers should prioritize suppliers with GMP-compliant synthesis facilities for consistent quality. Key specifications to verify include HPLC purity (>90%), endotoxin levels (<0.1 EU/μg for in vivo use), and absence of single-stranded contaminants. Custom sequence services typically offer length options from 20-1000 bp, with prices increasing for longer fragments. Bulk purchasing (10+ mg) often reduces per-unit costs by 30-50%. Consider vendors providing QC data including gel electrophoresis and mass spec verification.
Related Manufacturers
- 主营：染色液、新材料、水苏糖、小鼠双链DNA、阻燃剂、氮化锶、蒽醌紫、氧氟酸、胎盘肽、喹烯酮、钒酸铵、硫酸锂、钒酸铈、硫酸锶、降钙素、紫苏烯、锂电池、布洛芬、丁酸铝、锑酸钠、烟酰胺、牛磺酸、褪黑素、鸡蛋白、磷酸铁、丙酸铵
- 主营：科研试剂盒、酶免试剂盒、elisa试剂盒、小鼠抗双链DNA抗体IgG、生化试剂盒、细胞、生物试剂、进口ELISA试剂盒、PCR试剂盒、生化测试盒
- 主营：小鼠抗双链DNA抗体、试剂盒、ELISA试剂盒、ELISA检测试剂盒
- 主营：科研试剂盒、酶免试剂盒、elisa试剂盒、小鼠抗双链DNA抗体IgG、细胞、生物试剂、进口ELISA试剂盒、PCR试剂盒、生化测试盒
- 主营：试剂盒、人类风湿因子、微生物细胞色素、小鼠结肠癌细胞、细胞、ELISA检测试剂盒、原代培养细胞、类风湿检测试剂、酶联免疫试剂盒、微生物检测试剂、抗体检测试剂盒、炎症因子试剂盒、血清检测试剂盒、肿瘤标志物试剂、真菌检测试剂盒、生化分析试剂盒、HEC-1-A 细胞、细胞株、肺癌体外细胞模型、乳腺肿瘤细胞株、多发性骨髓瘤细胞、肠道癌细胞系、髓母细胞瘤冻存株、乳腺肿瘤细胞、结肠癌细胞系
- 主营：小鼠抗双链DNA抗体、试剂盒
- 主营：scl-70igg、人1α羟、试剂盒、小鼠抗双链DNA抗体、葡聚糖、大鼠11β、16α羟基、1iga抗体、胶原c端、2-he2elisa、1igm抗体、25二羟基、17羟孕酮、dna糖苷酶、5α还原酶、研启生物、去氢血栓、人胰岛素、胶原n末端、大鼠8羟基、25羟基维生、2，5寡腺苷、大鼠25羟基、11脱氢血栓、羟基壬烯醛、三磷酸肌醇
- 主营：ATCC细胞系、原代细胞、原代永生化
- 主营：抗双链DNA、elisa试剂盒
- 主营：ELISA试剂盒、PCR试剂盒、生化试剂盒、小鼠抗双链DNA抗体、科研试剂
- 主营：ELISA试剂盒、大鼠ELISA试剂盒、生化检测试剂盒、小鼠ELISA试剂盒、酶联免疫检测试剂盒、人ELISA检测试剂盒、植物检测试剂盒、兔ELISA试剂盒、猪elisa检测试剂盒、犬ELISA试剂盒、科研ELISA检测试剂盒
- 主营：ELISA检测试剂盒
- 主营：抗双链DNA抗体、elisa试剂盒
- 主营：试剂盒、反应蛋白、细胞、抗双链DNA抗体、抗体、酶免试剂盒
- 主营：抗双链DNA、elisa试剂盒、pcr检测试剂盒