Overview
DNP Amine, or 2,4-Dinitrophenylhydrazine (DNPH), is a chemical reagent primarily used in analytical chemistry. It is known for its ability to react with carbonyl compounds (aldehydes and ketones) to form brightly colored derivatives, making it invaluable for identification and quantification purposes. First synthesized in the late 19th century, DNPH has become a standard tool in laboratories worldwide. Its distinctive orange-red crystals and high reactivity make it easily identifiable. The compound's stability and specificity for carbonyl groups have cemented its role in both academic and industrial settings.
Physical and Chemical Properties
DNP Amine appears as orange to red crystalline powder with a molecular weight of 198.14 g/mol. It has a density of 1.63 g/cm³ and melts with decomposition at 198-200°C. The compound is soluble in organic solvents like ethanol and acetone but only slightly soluble in water. Chemically, DNPH is highly reactive with carbonyl compounds, forming hydrazone derivatives that are often yellow, orange, or red. This reaction is the basis for its use in qualitative and quantitative analysis. The compound should be handled with care due to its toxicity and potential to cause irritation upon contact.
Main Applications
The primary application of DNP Amine is in the detection and analysis of aldehydes and ketones. It is widely used in HPLC (High-Performance Liquid Chromatography) and spectrophotometric methods to quantify carbonyl compounds in various samples, including environmental, pharmaceutical, and food products. In addition to analytical chemistry, DNPH is employed in the synthesis of other chemical compounds and as a derivatizing agent to improve the detection limits of certain analytes. Its specificity and reliability make it a preferred choice in quality control laboratories and research institutions.
Safety and Storage
DNP Amine is toxic if ingested or inhaled and can cause skin and eye irritation. Proper personal protective equipment (PPE), including gloves, goggles, and lab coats, should be worn when handling this chemical. Adequate ventilation is essential to prevent inhalation of dust. Storage conditions are critical for maintaining the compound's stability. DNPH should be kept in a cool, dry place, away from oxidizing agents and acids. Containers must be tightly sealed to prevent moisture absorption and degradation. Spills should be cleaned up immediately using appropriate absorbent materials.
B2B Procurement Guide
When procuring DNP Amine, buyers should verify the purity grade required for their specific application. Technical grade (90-95% purity) is suitable for general laboratory use, while higher purity grades (98%+) are necessary for sensitive analytical methods. Reliable suppliers should provide a Material Safety Data Sheet (MSDS) and a certificate of analysis (CoA) with each batch. Bulk purchases may offer cost savings, but storage capacity and shelf life should be considered. Prices typically range from $50 to $100 per 100g, depending on purity and supplier.
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