Overview
Diphosphoglyceric acid (2,3-DPG) is an organic phosphate compound that serves as a critical metabolic intermediate in the glycolytic pathway. First isolated from erythrocytes in 1925, this molecule plays a central role in regulating oxygen transport by modulating hemoglobin's oxygen-binding affinity. In industrial contexts, 2,3-DPG is primarily used as a biochemical reagent for research on cellular metabolism and blood physiology. Its commercial production typically involves enzymatic synthesis or extraction from biological sources, with strict quality control for research applications.
Physical and Chemical Properties
As a phosphorylated carboxylic acid, 2,3-DPG exhibits high water solubility due to its multiple polar functional groups. The molecule is relatively unstable at room temperature, gradually hydrolyzing into simpler phosphates. Its crystalline form is hygroscopic, requiring careful moisture control during storage. The compound's most distinctive chemical property is its ability to bind reversibly to deoxyhemoglobin, reducing oxygen affinity through allosteric modulation. This interaction occurs through specific binding sites in the hemoglobin tetramer, making 2,3-DPG concentration crucial for proper oxygen delivery in tissues.
Main Applications
In medical research, 2,3-DPG is essential for studying oxygen transport dynamics and developing blood storage solutions. It helps maintain erythrocyte functionality in blood banks by preventing excessive oxygen binding during storage. The compound also finds use in diagnostic kits for metabolic disorders and as a reference standard in chromatography. Industrial applications include enzyme studies and as a component in specialized cell culture media where oxygen regulation is critical.
Safety and Storage
As a moderately acidic compound, 2,3-DPG requires handling with nitrile gloves and eye protection to prevent irritation. Powder forms may become airborne during handling, necessitating fume hood use or respiratory protection for large quantities. For long-term stability, aliquots should be stored at -20°C in sealed containers with desiccant. Aqueous solutions are typically stable for weeks when refrigerated at 4°C and buffered near physiological pH (7.2-7.4). Avoid repeated freeze-thaw cycles which accelerate degradation.
B2B Procurement Guide
When sourcing 2,3-DPG for commercial use, verify the certificate of analysis for purity (typically 95-98%), residual solvent content, and endotoxin levels for biomedical applications. Bulk purchases (100g+) may qualify for 15-30% discounts from specialty chemical suppliers. Consider whether the sodium or potassium salt form better suits your application, as this affects solubility and compatibility with biological systems. Lead times for custom syntheses can range 4-8 weeks, so plan procurement accordingly for time-sensitive projects.
Related Manufacturers
- 主营：蛋白酶、蜗牛酶、心肌黄酶、肌酸激酶、亮抑肽酶、淀粉酶、乳糖酶、亮抑酶肽、硫代辅酶、细胞溶解酶、果胶酶、纤维素酶、枯草杆菌、乙醇脱氢酶、木聚糖酶、氯化溶菌酶、脂氧素、葡球菌酶、羧化辅酶、尿素酶、氯化酵素
- 主营：合成酶、微量法、dna提取、人叶酸、雄二酮、糖蛋白、试剂盒、脂蛋白、人落叶、测试盒、果汁中、dnamarker、人新生、模拟abo、dna抗体、赫澎人、极速pcr、人免疫、病毒igg、双链dna、人睾酮、人多肽、干扰素、食欲素、人血管
- 主营：二乙烯三胺回收、过期环氧树脂
- 主营：回收树脂、回收油漆、回收颜料、回收硬脂酸、回收硬脂酸锌、回收碳酸钾、回收黄原胶、回收瓜尔胶、回收化工原料、回收氧化锌、回收橡胶、回收促进剂、回收热熔胶、回收真石漆、回收钛白粉、回收乳液、回收聚氯乙烯树脂、回收纤维素、回收珠光粉、回收香精、回收导热油、回收赤藓糖醇、回收表面活性剂、回收日化原料