Overview
Mouse Dihydropyrimidinase-like Protein 2 (DPYSL2), also known as Collapsin Response Mediator Protein-2 (CRMP-2), is a cytosolic phosphoprotein that plays critical roles in neuronal development and differentiation. It belongs to the dihydropyrimidinase-related protein family and is highly conserved across species. DPYSL2 is particularly abundant in the nervous system, where it participates in axonal guidance, growth cone collapse, and neurite outgrowth through its interaction with tubulin heterodimers and microtubule dynamics. Originally identified as a downstream effector of Semaphorin 3A signaling, this protein has become an important research target in neurobiology. Its expression patterns change significantly during brain development, making it a valuable marker for studying neural circuit formation. The protein's multifunctional nature extends to synaptic plasticity regulation, contributing to its relevance in both developmental neuroscience and neurodegenerative disease research.
Physical and Chemical Properties
DPYSL2 typically exists as a homotetramer in its functional state, with each monomer weighing approximately 62-66 kDa depending on post-translational modifications. The protein demonstrates optimal stability in neutral pH buffers (pH 7.0-7.4) and requires reducing agents like DTT to maintain cysteine residues in their reduced state. Its secondary structure contains significant α-helical content, which is crucial for its interaction with microtubules. The protein's solubility depends on buffer composition, with phosphate-buffered saline (PBS) or Tris-based buffers commonly used for storage. DPYSL2 is susceptible to proteolytic degradation unless stored at -20°C or below with protease inhibitors. Its isoelectric point falls within the slightly acidic range (pH 5.5-6.5), which influences its behavior during protein purification procedures such as ion-exchange chromatography.
Main Applications
In neuroscience research, DPYSL2 serves three primary purposes: as a neuronal differentiation marker, a tool for studying axonal guidance mechanisms, and a potential therapeutic target for neurological disorders. Researchers utilize antibodies against DPYSL2 in immunohistochemistry to visualize developing neurons and assess neurodevelopmental processes in mouse models. The protein's phosphorylation status provides insights into Semaphorin signaling pathways. Pharmaceutical studies investigate DPYSL2's role in neurodegenerative diseases such as Alzheimer's, where its abnormal phosphorylation correlates with neurofibrillary tangle formation. Recent applications extend to neuroregeneration research, particularly in spinal cord injury models. Biotechnology companies often include recombinant mouse DPYSL2 in kits for neuronal cell culture and differentiation studies, capitalizing on its ability to influence microtubule dynamics in vitro.
Safety and Storage
As a research-grade biological product, mouse DPYSL2 requires standard biosafety level 1 (BSL-1) handling precautions. Users should wear appropriate personal protective equipment including gloves and lab coats. Although not classified as hazardous, the protein should be treated as a potential irritant, avoiding contact with skin and eyes. For optimal preservation, lyophilized preparations should be stored at -20°C in a desiccated environment, while liquid formulations require aliquoting to prevent freeze-thaw cycles. Adding glycerol (10-50%) can enhance stability for long-term storage at -80°C. Researchers should note that repeated thawing may lead to protein aggregation, which can be checked by SDS-PAGE or dynamic light scattering. Proper storage typically maintains protein integrity for 12-24 months.
B2B Procurement Guide
When procuring mouse DPYSL2 for research purposes, buyers should prioritize suppliers with demonstrated experience in neuronal protein production. Key specifications to verify include: ≥90% purity (SDS-PAGE verified), endotoxin levels (<1 EU/μg), and functional validation data (typically neurite outgrowth assays). Bulk purchasers should request lot-specific certificates of analysis. Price negotiations often become favorable for orders exceeding 5mg, with academic discounts commonly available. Lead times vary (2-6 weeks) depending on customization requirements such as specific tags (His, GST) or phosphorylation status. For cell culture applications, consider purchasing carrier proteins (e.g., BSA) to prevent adsorption losses. Reliable suppliers typically provide technical support for troubleshooting experimental protocols, which adds value beyond the product itself.
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