Deep Red Fluorescent Probe

Updated: 2026-09-15

Overview

The Deep Red Fluorescent Probe is a highly sensitive chemical tool designed for fluorescence-based detection in biological and chemical research. Its deep red emission (typically 650-700 nm) minimizes autofluorescence interference, making it suitable for complex samples like tissues or cellular structures. These probes are often derived from cyanine or other fluorophore families, engineered for brightness and stability. Commonly used in conjunction with microscopy or flow cytometry, these probes enable researchers to track molecular processes, label specific targets, or monitor dynamic interactions in real time. Their low cytotoxicity and tunable properties make them versatile for live-cell imaging and diagnostic applications.

Physical and Chemical Properties

Deep Red Fluorescent Probes exhibit strong absorption and emission in the far-red to near-infrared spectrum, reducing scattering and improving signal-to-noise ratios in thick samples. Their photostability allows prolonged imaging without significant photobleaching, a critical feature for time-lapse studies. Solubility varies by formulation, but most probes dissolve well in dimethyl sulfoxide (DMSO) or ethanol, with aqueous compatibility often enhanced by chemical modifications. Storage at -20°C in dark conditions is recommended to prevent degradation. Some formulations include reactive groups (e.g., NHS esters) for covalent labeling of biomolecules.

Main Applications

These probes are pivotal in cell biology for staining organelles, tracking proteins, or monitoring ion concentrations (e.g., calcium sensors). In diagnostics, they serve as labels for immunoassays or in situ hybridization, leveraging their spectral separation from common green/yellow fluorophores. In drug discovery, Deep Red Fluorescent Probes facilitate high-throughput screening by enabling multiplexed assays. Their near-infrared emission is also exploited in preclinical imaging, where deeper tissue penetration is required. Customizable derivatives are increasingly used in super-resolution microscopy to push resolution limits.

Safety and Storage

Handling requires standard laboratory precautions: use gloves, goggles, and work in a fume hood if volatile solvents are involved. Probes in powder form may pose inhalation risks, so weigh in closed systems. Dispose of waste according to local regulations for organic compounds. Long-term stability depends on proper storage. Lyophilized powders should be kept desiccated, while liquid stocks benefit from aliquoting to avoid freeze-thaw cycles. Shield from light during use and storage to preserve fluorescence efficiency. Check manufacturer datasheets for specific stability data.

B2B Procurement Guide

When sourcing Deep Red Fluorescent Probes, prioritize suppliers with documented quality control (e.g., HPLC purity certificates, mass spectrometry validation). Batch-to-batch consistency is critical for reproducible experiments. Request emission/excitation spectra to confirm compatibility with your equipment. Bulk purchases may offer cost savings, but verify storage feasibility. For specialized applications (e.g., two-photon excitation), inquire about custom modifications. Lead times can vary; confirm availability if working under deadlines. Some suppliers provide technical support for probe selection and protocol optimization.