Overview
DBCO-Cy5 is a bifunctional compound combining the near-infrared fluorescent dye Cy5 with a dibenzocyclooctyne (DBCO) group. The DBCO moiety enables strain-promoted azide-alkyne cycloaddition (SPAAC), a copper-free click chemistry reaction, while Cy5 provides strong fluorescence for detection. This combination makes it valuable in live-cell imaging and biomolecule tracking. Developed as an alternative to traditional labeling methods, DBCO-Cy5 offers faster reaction kinetics and reduced cytotoxicity compared to copper-catalyzed click chemistry. Its stability and sensitivity have made it a preferred tool in pharmaceutical research and diagnostic assay development.
Physical and Chemical Properties
The compound exhibits a dark blue to purple appearance due to the Cy5 chromophore. It is typically supplied as a lyophilized powder with high purity (>95%). The DBCO group reacts selectively with azides at physiological pH and temperature, while the Cy5 dye absorbs maximally at ~650 nm and emits at ~670 nm, ideal for minimizing background interference in biological samples. Solubility is limited in aqueous buffers but excellent in polar organic solvents like DMSO or DMF. Stability tests indicate degradation under prolonged light exposure or at temperatures above 40°C, necessitating strict storage protocols.
Main Applications
DBCO-Cy5 is extensively used in fluorescence microscopy and flow cytometry to label azide-modified biomolecules, such as glycoproteins or cell-surface receptors. Its near-infrared emission reduces autofluorescence in tissues, enabling deep-tissue imaging applications. In drug discovery, it serves as a tracer for target engagement studies or antibody-drug conjugate (ADC) characterization. The compound also facilitates the development of diagnostic probes, particularly in multiplex assays where multiple dyes are required for simultaneous detection.
Safety and Storage
As a light-sensitive compound, DBCO-Cy5 requires protection from direct sunlight and prolonged exposure to ambient light. Recommended storage is at -20°C under argon or nitrogen to prevent oxidation. Aliquoting is advised to avoid repeated freeze-thaw cycles. Safety data sheets classify it as harmful if inhaled or ingested. Laboratory handling should include gloves, safety goggles, and fume hoods. Spills must be contained with inert absorbents and disposed of as hazardous organic waste.
B2B Procurement Guide
When sourcing DBCO-Cy5, prioritize suppliers with ISO-certified manufacturing facilities. Key specifications to verify include HPLC purity (>95%), absence of free Cy5 dye impurities, and moisture content (<1%). Batch-to-batch consistency is critical for reproducibility in long-term studies. Bulk purchasing (100 mg+) may reduce costs by 15–30%, but validate stability claims for larger quantities. Some vendors offer custom conjugation services (e.g., peptide-DBCO-Cy5 constructs), which can streamline downstream applications. Lead times for specialty orders typically range from 2–6 weeks.
Related Manufacturers
- 主营：cy荧光染料、ICG吲哚菁绿、源头厂家
- 主营：fitc标记蛋白、聚苯乙烯微球、pvc微球、生物素修饰蛋白、嵌段共聚物、生物素标记、荧光标记、纳米颗粒、核苷糖、aie、复合微球、功能化载玻片、荧光染料、金属配合物、Mxene、介孔二氧化硅微球、磁性纳米颗粒、小分子PEG、聚合物、纳米金、聚合物微球、荧光微球、荧光探针、点击化学、小分子
- 主营：反应活、hydrazide、催化剂、修饰剂、糖蛋白、连接子、激动剂、聚合物、活性脂、胆固醇、钆特酸、催化性、dna标记、络合剂、荧光试剂、雨蛙素、乙二胺、ped结构、衍生物、化合物、链接剂、反应物、生物素
- 主营：催化剂、peg试剂、活性脂、cy5四氮、环辛炔、苯二胺、2406-34-0、乙氧基、试剂cas、环辛烯、生物素、cb-cyclam、实验室、dbco-acid、半乳糖、溴苯基、交联剂、环己炔、荧光素、3694-51-7、ac4galnal、2001-96-9、葡萄糖、直发cas、糖化学
- 主营：在水中、包含肽、含叠氮、丙炔基、由氨基、菁染料、peg单元、具有炔、胺hcl盐、阿尔法、催化剂、peg试剂、结构式、氨基nh2、3694-51-7、碘化物、4727-50-8、具有两、带有炔、磺酸基、3065-79-0、aminopeg8、甲基九、bcn基团、荧光素
- 主营：甲氧基聚乙二醇、氨基聚乙二醇、羧基聚乙二醇、巯基聚乙二醇、羟基聚乙二醇、马来酰亚胺聚乙二醇、四臂聚乙二醇、荧光素聚乙二醇、叠氮聚乙二醇、硅烷聚乙二醇、炔基聚乙二醇、生物素聚乙二醇、丙烯酸酯聚乙二醇、活性脂聚乙二醇、Boc保护基团聚乙二醇、Fmoc保护基团聚乙二醇、生物素小分子PEG衍生物、甲氧基小分子PEG衍生物
- 主营：二苯基环辛炔、莪术二酮、人参皂苷rc
- 主营：单分散peg、聚乙二醇、马来酰亚胺
- 主营：磷脂聚乙二醇、胆固醇聚乙二醇、PLGA、功能化聚乙二醇、硬脂酸聚乙二醇、透明质酸、聚乙内酯、聚乳酸、葡聚糖、DOPE
- 主营：嵌段共聚物、合成磷脂、荧光染料、Cy5二苯基环辛炔、PEG、点击化学、量子点
- 主营：cy5、纳米颗粒、脂质体
- 主营：金纳米、三角片、麦芽糖、修饰tio2、羟丙基、ito薄膜、氮化钛、tio2纳米、碳粉tio2、pd纳米笼、水溶伊红、纳米复合、磁性纳米、蛋白芯片、包硅金银、硅纳米线、包硅纳米、硅纳米管、螯合树脂、油溶性银、复合材料、薄膜定制、纳米粒子