Overview
Dabcyl-DBCO is a specialized chemical reagent that combines two important functional groups: the fluorescence quencher Dabcyl and the strained alkyne DBCO (dibenzocyclooctyne). This bifunctional compound enables efficient bioorthogonal labeling through copper-free click chemistry reactions with azide-containing molecules. The reagent is particularly valuable in biological research where simultaneous quenching and labeling are required. Its design allows for specific, rapid conjugation without toxic copper catalysts, making it suitable for live-cell applications and sensitive biological systems.
Physical and Chemical Properties
Dabcyl-DBCO typically appears as a dark red to brown solid with good stability when stored properly. The compound exhibits excellent solubility in polar organic solvents such as DMSO and DMF, but limited solubility in aqueous buffers unless modified with hydrophilic groups. The Dabcyl moiety provides strong fluorescence quenching properties, typically absorbing in the 450-550 nm range, while the DBCO group enables rapid, specific reactions with azides through strain-promoted alkyne-azide cycloaddition (SPAAC). This combination creates a versatile tool for designing various detection and labeling systems.
Main Applications
The primary application of Dabcyl-DBCO is in developing fluorescence-quenched probes for biological detection. Researchers utilize it to create molecular beacons, FRET-based sensors, and other diagnostic tools where controlled signal generation is required. In drug discovery, Dabcyl-DBCO serves as a valuable linker for creating targeted conjugates. The DBCO group allows specific attachment to azide-modified biomolecules, while the Dabcyl moiety can provide quenching functionality or serve as a spectroscopic handle. Additional applications include surface modification, nanoparticle labeling, and the development of bioimaging probes.
Safety and Storage
As with many chemical reagents, proper handling procedures should be followed when working with Dabcyl-DBCO. Standard personal protective equipment including gloves, lab coat, and eye protection is recommended. The compound should be used in a well-ventilated area or chemical fume hood. For long-term storage, Dabcyl-DBCO should be kept at -20°C in a tightly sealed container, protected from light and moisture. The solid is typically stable for at least 12 months when stored properly, while solutions in DMSO or other solvents should be used within days or weeks depending on concentration and storage conditions.
B2B Procurement Guide
When procuring Dabcyl-DBCO for research or industrial applications, several factors should be considered. First, verify the purity specification (typically ≥95% by HPLC) and request certificate of analysis from the supplier. The presence of free DBCO or Dabcyl impurities can affect performance in sensitive applications. For bulk purchases, inquire about custom packaging options to minimize exposure to air and moisture during storage. Consider suppliers who provide detailed technical support and can supply characterization data (NMR, HPLC, MS). Lead times may vary significantly depending on the supplier's inventory and synthesis schedule.
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