Overview
Human Cyclophilin B (CypB) is a ubiquitously expressed immunophilin with peptidyl-prolyl cis-trans isomerase (PPIase) activity. It is involved in protein folding, particularly in the endoplasmic reticulum, and interacts with cyclosporin B, a derivative of the immunosuppressive drug cyclosporin A. CypB also plays roles in immune response modulation and viral replication, making it a target for therapeutic research. CypB is encoded by the PPIB gene and is part of a larger family of cyclophilins. Its structure includes a conserved domain that facilitates its PPIase activity, which is critical for the proper folding of proteins containing proline residues. This protein is widely studied in the context of autoimmune diseases, viral infections, and cancer.
Physical and Chemical Properties
Human Cyclophilin B is a small protein with a molecular weight of approximately 23 kDa. It typically appears as a white to off-white lyophilized powder and is soluble in aqueous buffers. The protein’s PPIase activity is its most notable chemical property, enabling it to catalyze the cis-trans isomerization of peptide bonds preceding proline residues. CypB is stable under recommended storage conditions (-20°C, desiccated) but may degrade if exposed to repeated freeze-thaw cycles or extreme pH levels. Its solubility and activity are pH-dependent, with optimal performance observed in neutral to slightly alkaline conditions.
Main Applications
Human Cyclophilin B is primarily used in biomedical research, particularly in studies related to protein folding, immune regulation, and viral replication. Its interaction with cyclosporin B makes it a valuable tool for investigating immunosuppressive mechanisms and developing novel therapeutics. In drug development, CypB is studied for its potential role in treating autoimmune diseases, such as rheumatoid arthritis, and viral infections, including HIV and hepatitis C. Additionally, its involvement in cancer progression has sparked interest in targeting CypB for oncology research.
Safety and Storage
When handling Human Cyclophilin B, standard laboratory precautions should be followed, including the use of gloves and protective eyewear. Avoid inhalation or direct contact with skin and eyes. In case of exposure, rinse thoroughly with water and seek medical advice if irritation persists. For long-term stability, store CypB at -20°C in a desiccated environment. Avoid repeated freeze-thaw cycles, as this may degrade the protein. Reconstituted solutions should be used immediately or stored at 4°C for short-term use (up to one week).
B2B Procurement Guide
When procuring Human Cyclophilin B for B2B purposes, prioritize suppliers with demonstrated expertise in protein production and purification. Key considerations include purity (≥95% recommended), endotoxin levels, and batch-to-batch consistency. Request certificates of analysis (CoA) to verify product specifications. Pricing varies depending on purity, quantity, and supplier reputation. Bulk purchases may qualify for discounts, but ensure storage capacity aligns with product stability requirements. Evaluate supplier reliability, shipping conditions, and customer support to mitigate procurement risks.
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