Overview
Cyclohexylaminoethanesulfonic Acid (CHES) is an organic sulfonic acid compound widely recognized for its buffering properties in the pH range of 9.3 to 10.7. It is commonly used in biochemical research and pharmaceutical manufacturing due to its stability and minimal interference with biological systems. The compound was first synthesized in the mid-20th century as part of efforts to develop improved buffer systems for biochemical applications. Its unique combination of a cyclohexylamine group and sulfonic acid moiety gives it distinct chemical properties that make it particularly useful in alkaline pH conditions.
Physical and Chemical Properties
CHES appears as a white crystalline powder with good solubility in water (approximately 50 g/L at 25°C) and limited solubility in organic solvents like ethanol. Its molecular structure features both hydrophilic (sulfonic acid) and hydrophobic (cyclohexyl) components, contributing to its amphiphilic nature. The compound demonstrates excellent chemical stability in aqueous solutions within its effective pH range, with minimal degradation over time. Its pKa of 9.55 at 20°C makes it particularly valuable for maintaining stable pH conditions in biochemical assays and diagnostic applications where alkaline conditions are required.
Main Applications
The primary use of CHES is as a biological buffer in molecular biology research, particularly in enzyme studies, electrophoresis, and cell culture applications requiring pH 9-10 conditions. It's favored over traditional buffers like glycine in applications where metal ion chelation must be minimized. In pharmaceutical manufacturing, CHES serves as an intermediate in the synthesis of certain drugs and as a stabilizing agent in formulations. The diagnostic industry utilizes it in various test kits and reagents, especially those involving alkaline phosphatase and other enzymes active in alkaline environments.
Safety and Storage
While CHES is not classified as highly hazardous, proper safety precautions should be followed. The powder can cause irritation to respiratory system, skin, and eyes. Appropriate personal protective equipment including gloves, goggles, and dust masks should be used when handling. For long-term storage, CHES should be kept in tightly sealed containers in a cool (15-25°C), dry environment away from strong oxidizers. Solutions should be prepared with deionized water and stored at 4°C for extended use, with regular pH verification. Shelf life of the dry powder is typically 3-5 years when stored properly.
B2B Procurement Guide
When procuring CHES for industrial or research purposes, buyers should specify required purity (typically 99% or higher for research applications), packaging size (from 100g to 25kg drums), and whether sterile processing is needed. Technical grade (95-98%) may be acceptable for some manufacturing uses at lower cost. Key procurement considerations include verifying the supplier's quality control documentation (including HPLC purity analysis), requesting current COA, and confirming packaging meets your storage requirements. Bulk buyers should inquire about volume discounts and lead times, as production may be batch-specific. Consider requesting a small test sample before large orders to verify performance in your specific application.
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