Overview
CY7-Azide is a functionalized cyanine dye featuring an azide (-N₃) group for click chemistry applications. As part of the CY series, it emits in the near-infrared (NIR) range, making it valuable for deep-tissue imaging with minimal background interference. The azide group enables selective conjugation to alkyne-modified biomolecules via copper-catalyzed azide-alkyne cycloaddition (CuAAC). Developed as a successor to visible-range dyes, CY7-Azide addresses the need for low-autofluorescence probes in complex biological systems. Its NIR properties are particularly useful for in vivo studies, where longer wavelengths penetrate tissues more effectively than visible light.
Physical and Chemical Properties
CY7-Azide exhibits strong absorbance at ~750 nm and emission at ~773 nm, characteristic of heptamethine cyanine dyes. The conjugated polymethine chain provides photostability, though prolonged light exposure should be avoided. Its azide group reacts efficiently with alkynes under mild conditions (room temperature, aqueous buffers). The dye shows moderate solubility in polar organic solvents but limited water solubility without solubilizing groups. Formulations often include cosolvents like DMSO for biological applications. Spectral properties remain stable across pH 5-9, making it compatible with physiological conditions.
Main Applications
In biomedical research, CY7-Azide is primarily used for labeling proteins, antibodies, and nucleic acids via click chemistry. Its NIR fluorescence enables real-time tracking of drug delivery systems and cellular processes in animal models. The dye is also employed in multiplex assays, where its emission doesn't overlap with common visible fluorophores. Industrial applications include quality control in biomanufacturing, where azide-tagged molecules are monitored during production. Some diagnostic kits incorporate CY7-Azide for high-sensitivity detection of low-abundance biomarkers in clinical samples.
Safety and Storage
As a dry powder, CY7-Azide is stable for years when stored at -20°C in amber vials with desiccants. Solutions in DMSO should be aliquoted to avoid freeze-thaw cycles and used within weeks. Decomposition is indicated by color fading or precipitation. Safety protocols mandate gloves and eye protection due to potential irritant effects. Although not acutely toxic, prolonged skin contact should be avoided. Spills require ethanol-based cleanup followed by water rinsing. Always consult the SDS for specific handling instructions.
B2B Procurement Guide
When sourcing CY7-Azide, prioritize suppliers providing analytical certificates with HPLC purity (>95%) and mass spectrometry verification. Bulk orders (1g+) may qualify for 15-30% discounts but require stability testing upon receipt. Some manufacturers offer custom modifications like sulfonation for improved water solubility. Lead times typically range from 2-6 weeks for specialty batches. Consider requesting a small trial quantity to validate performance in your specific application before large-scale purchase. Logistics should ensure cold chain transportation to maintain product integrity.
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