Culture Medium Solidifying Agent
Overview
Culture medium solidifying agents are essential components in microbiological and cell culture laboratories. They provide the structural support needed for microbial or cellular growth by converting liquid media into semi-solid gels. The most common agents include agar, derived from seaweed, and gelatin, sourced from animal collagen. These agents are selected for their ability to form stable, inert matrices that do not interfere with biological processes. Agar is preferred for its higher melting point, making it suitable for incubations at 37°C, while gelatin is used in specific applications requiring lower gelation temperatures.
Physical and Chemical Properties
Agar, the dominant solidifying agent, exhibits unique thermoreversible properties: it melts at 85-90°C and solidifies at 32-45°C. This allows easy preparation and sterilization of media without losing gelling capacity. Its polysaccharide structure ensures chemical inertness, preventing interactions with nutrients or metabolites. Gelatin, a protein-based alternative, forms gels at lower temperatures (20-25°C) but melts around 35°C, limiting its use in warmer incubations. Both agents are odorless, tasteless, and typically supplied as dehydrated powders or granules for convenient storage and handling.
Main Applications
In microbiology, solidifying agents enable colony isolation and enumeration on Petri dishes, critical for research, diagnostics, and pharmaceutical quality control. They are also used in plant tissue culture to support explant growth and in food microbiology for pathogen detection. The food industry utilizes these agents as thickeners or stabilizers in products like desserts and canned meats. Bacteriological-grade agar is mandatory for lab use to avoid contaminants, while food-grade variants suffice for culinary applications. Specialty formulations may include added nutrients or antibiotics for selective media.
Safety and Storage
Culture medium solidifying agents are generally safe but require standard lab precautions. Powder forms can generate dust, necessitating masks during handling to prevent respiratory irritation. Spills should be cleaned promptly to avoid slip hazards. Storage recommendations include airtight containers in low-humidity environments to prevent clumping. Agar and gelatin are stable at room temperature but may degrade if exposed to prolonged heat or moisture. Shelf life typically exceeds two years when stored properly, though performance should be verified for critical applications.
B2B Procurement Guide
Industrial buyers should prioritize suppliers with ISO or GMP certifications to ensure consistency and traceability. Key specifications include gel strength (e.g., 800-1200 g/cm² for standard agar), ash content (<5%), and microbiological purity (e.g., <300 CFU/g). Bulk purchases (25+ kg) often reduce costs by 15-30%. Consider regional logistics—agar is predominantly sourced from maritime Southeast Asia, while gelatin suppliers are global. Request certificates of analysis (CoA) for each batch, and validate performance with small-scale trials before large orders.
Related Manufacturers
- 主营：丙酸钙、肉桂酸、维生素C/抗坏血酸、培养基琼脂粉厂家、edta二钠、纳他霉素、抗氧剂264、抗坏血酸、双乙酸钠、聚赖氨酸、胍基乙酸、抗氧剂t501、抗氧剂1010、抗氧剂dltp、半胱氨酸盐酸盐、半胱氨酸、果胶、干酪素、苯丙氨酸、乳酸链球菌素、肉桂酸钾、色氨酸、牛磺酸、低聚木糖、酪蛋白酸钠、维生素C、vc
- 主营：菌检测、菌落计数器、水质检测、培养基、培养皿、ms培养基、支原体培养基、路桥培养基、马铃薯葡萄糖琼脂培养基、三药培养基、海博培养基、微生物检测仪器设备、接种环、微生物检验、微生物检测、程控定量封口机、菌种鉴定
- 主营：木糖醇、乳糖、木糖、培养基琼脂粉厂家、胍基乙酸、黄原胶、海藻酸钠、酪蛋白酸钠、酪蛋白/干酪素、甘氨酸、牛磺酸、瓜尔胶/瓜尔豆胶、果胶/苹果果胶、维生素CL-抗坏血酸、聚赖氨酸、苯丙氨酸、半胱氨酸、苯甲地那铵 苦精、蔗糖八乙酯 苦味剂、甘露糖醇/甘露醇、海藻糖、山梨糖醇/山梨醇、卡拉胶、蛋氨酸
- 主营：甘氨酸、水苏糖、阻燃剂、凝固剂、硫酸锌、硫酸锆、硫酸锂、钒酸铋、硫酸锰、梅栲胶、溴氨酸、聚合物、锂电池、牛磺酸、磷酸铁、磷酸铋、磷酸铌、碳酸镁、碳酸镍、肉桂醛、防冻剂、磷酸铝、新癸酸、椰油胺、辛癸醇、剥色剂
- 主营：铝粒、管道疏通剂、彩漂粉、凝固剂、爆炸盐、过碳酸钠、香精、彩色蛋白酶、增稠剂、甘油、硼酸、丁二酸、十二烷基苯磺酸钠、洗衣粉母粉、AES、NP-10、AEO~9、6501、磺酸、CAB-35、LAo~30
- 主营：黄原胶、海藻酸钠、酪蛋白酸钠、培养基琼脂粉厂家、胍基乙酸、瓜尔胶、聚丙烯酸钠、栀子黄、柑橘黄、红花黄、墨鱼汁粉、姜黄、叶绿素铜钠盐、魔芋粉、南瓜粉、紫薯粉、双乙酸钠、苯甲酸钠、丙酸钙、大豆分离蛋白、卡拉胶、阿拉伯胶、魔芋胶、乳清粉、乳清蛋白
- 主营：乳糖、木糖醇、黄原胶、凝固剂、维生素C、瓜儿豆胶、粉条增筋剂、海藻酸钠、苦味剂、乳酸链球菌素、木瓜蛋白酶、大豆异黄酮、甘露糖醇、果胶、魔芋粉、一水柠檬酸、牛黄酸、脱氢乙酸钠、山梨糖醇、亚硫酸氢钠、L一精氨酸、纳他霉素、薄荷脑
- 主营：食用香料、增稠稳定剂、食品增甜剂、增稠凝固剂、黄原胶增稠剂、高酰基结冷胶
- 主营：甘氨酸、阿力甜、酸酯镁、凝固剂、白色素、水苏糖、海藻糖、脆皮素、儿茶素、南瓜粉、五香粉、硫酸锰、肌氨酸、酪蛋白、牛磺酸、碳酸镁、吸湿剂、菊糖酶、卡拉胶、柚苷酶、乳糖酶、泛酸钙、氯化锌、乳酸钠、文莱胶、叶绿素
- 主营：染色液、乙氨酸、噻唑兰、凝固剂、喷雾剂、封片剂、清除剂、试剂盒、研试剂、转膜液、dpph试剂、page凝胶、te缓冲液、pbs缓冲液、检测溶液、核酸染料、中性树胶、胰蛋白酶、纤维素酶、染色试剂、mes缓冲液、faa固定液、甲苯胺蓝、生化试剂、实验试剂、ctab抽提液
- 主营：特种混凝土、铁砂混凝土、配重混凝土、凝固剂、铁矿石混凝土、防辐射混凝土、重晶石混凝土、耐高温混凝土、钢砂混凝土、桥梁配重、钢箱梁配重、硫酸钡混凝土、铁屑混凝土、铁屑砂浆、防辐射砂浆、耐高温砂浆、不发火混凝土、配重材料、含硼混凝土、钢珠混凝土、钢渣混凝土、钢箱梁铁砂混凝土、配重铁砂混凝土、铁砂压重混凝土、压重铁砂混凝土、桥梁铁砂混凝土
- 主营：乙酸铵、草酸铵、甲酸铵、凝固剂、氯化钾、氯化镁、乙酸钾、乙酸钠、醋酸钾、硫酸钠、碳酸钾、磷酸三钾、柠檬酸铵、焦磷酸钠、硫酸亚铁、硫酸镁、磷酸氢二钾、磷酸氢二钠、柠檬酸钾、甲酸钾、碳酸氢钾、磷酸三钠、磷酸二氢钠、硫酸钾、尿素、双乙酸钠
- 主营：氯化钾、柠檬酸铵、柠檬酸氢二铵、凝固剂、柠檬酸钾、磷酸氢二钾、磷酸二氢钾、磷酸钾、乙酸铵、乙酸钠、醋酸铵、醋酸钠、乙酸钾、草酸铵、醋酸钾、硫酸镁、硫酸锌、甲酸铵、尿素、草酸铜、草酸钾、硫酸锰、硫酸钠、硫酸铵、磷酸氢镁
- 主营：食品配料、酶制剂、提取物、凝固剂、有机硒、无机硒、有机化学原料、无机化学原料
- 主营：卡波姆、卡拉胶、可得然胶、凝固剂、魔芋粉 魔芋精粉、琼脂 琼脂粉、羧甲基纤维素钠、明胶、聚维酮K30、乙基纤维素、D-氨基葡萄糖盐酸盐、壳聚糖、壳寡糖、酪蛋白 干酪素、酪蛋白酸钠 酪朊酸钠、氯化胆碱、甜菜碱、酵母浸粉、蛋白胨、低聚果糖、低聚木糖、甘露寡糖、菊粉、D-异抗坏血酸钠
- 主营：工业柠檬酸、工业葡萄糖、过硫酸钠、凝固剂、聚丙烯酰胺、磷酸氢二钠、磷酸二氢钾
- 主营：L-半胱氨酸盐酸盐、L-谷氨酰胺、L-瓜氨酸、凝固剂、L-精氨酸、L-赖氨酸、L-酪氨酸、L-亮氨酸、L-脯氨酸、L-苏氨酸、L-缬氨酸、氨基丁酸、甘氨酸、胍基乙酸、支链氨基酸、酪蛋白酸钠、螺旋藻、维生素E、D-生物素、氨糖、一水肌酸、肌醇、菊粉、维生素A、维生素B1、柠檬酸
- 主营：凝固剂、低聚果糖
- 主营：渗透剂、十八胺、乳化剂、凝固剂、十二胺、匀染剂o、石油化工、十六十八醇、醇聚氧乙烯醚、乳百灵、聚丙二醇 PPG、脂肪酸酯、磷酸酯、匀染剂、甘油三油酸酯、辛基酚聚氧乙烯醚、聚醚、消泡剂、农药乳化剂、分散剂、添加剂AC、乳化剂司盘、抗静电剂、纺织前处理助剂
- 主营：EDTA-2Na、EDTA-4Na、草酸、凝固剂、草酸钠、纯碱、醋酸钠、防染盐、焦磷酸钠、磷酸二氢钾、六偏磷酸钠、麦芽糊精、尿素、柠檬酸、柠檬酸钠、硼砂、葡萄糖、葡萄糖酸钠、三水醋酸钠、液体醋酸钠、工业葡萄糖、液体草酸、结晶性粉、农业尿素、乙二胺四乙酸二钠、酒石酸
- 主营：指示卡、测试卡、紫外线强度检测卡、凝固剂、消毒液浓度检测试纸、水质检测试纸
- 主营：五氧化二钒、苄基胂酸、水杨酸、凝固剂、水玻璃、氨基磺酸、多聚甲醛
- 主营：云母粉、蛭石粉、膨润土、凝固剂、硅藻土、电气石、云母片、麦饭石、石英砂、重晶石、sio2焊条、发热铁砂、耐火漂珠、煅烧沸石、防火涂料、膨胀蛭石、空心漂珠、涂料彩砂、生蛭石片、白色蛭石、实木木粉、染色彩砂、火山熔岩、晶体电介质、滤料鹅卵石、生物质燃料
- 主营：防酶剂、表面活性剂、次氯酸钙、凝固剂、福美钠、蛋白胨、氟化氢钾、氟化钠、氟化钾、浮选油