Overview
Claudin-6 (CLDN6) is a member of the claudin family of tight junction proteins, which are essential components of epithelial and endothelial cell barriers. First identified in 2001, it plays specialized roles in tissue-specific barrier formation and cellular adhesion. As a tetraspan transmembrane protein, Claudin-6 forms the backbone of tight junction strands and selectively regulates paracellular transport. Its expression is predominantly found in embryonic tissues and certain carcinomas, making it a biomarker of interest in developmental biology and oncology research.
Physical and Chemical Properties
Claudin-6 has a molecular weight of approximately 23 kDa and consists of 220 amino acids with four transmembrane domains. The protein features two extracellular loops that mediate homotypic and heterotypic interactions with other claudins. The N-terminal domain contains sorting signals for tight junction localization, while the C-terminal PDZ-binding motif interacts with cytoplasmic scaffolding proteins. Recombinant Claudin-6 typically appears as a white lyophilized powder that remains stable for years when properly stored at -20°C or below.
Main Applications
In cancer research, Claudin-6 serves as a diagnostic marker for germ cell tumors and certain carcinomas. Its restricted expression pattern in adult tissues makes it an attractive target for antibody-drug conjugates and CAR-T therapies. The protein is also used in epithelial barrier studies, particularly in modeling intestinal and blood-brain barrier functions. Pharmaceutical researchers employ Claudin-6 in drug permeability assays to predict compound absorption across biological barriers.
Safety and Storage
Research-grade Claudin-6 poses minimal biological hazard but should be handled using standard laboratory precautions. Use gloves and eye protection when reconstituting lyophilized protein to prevent contamination. For long-term storage, maintain lyophilized protein at -20°C in desiccated conditions. Reconstituted solutions should be aliquoted and stored at -80°C with carrier proteins (e.g., 0.1% BSA) to prevent adsorption losses. Avoid repeated freeze-thaw cycles to preserve activity.
B2B Procurement Guide
When sourcing Claudin-6, verify the expression system (mammalian preferred for proper folding) and purity level (≥95% by SDS-PAGE). For functional studies, request certificates of analysis showing barrier formation activity. Bulk purchasers should negotiate batch-to-batch consistency guarantees. Consider suppliers offering customized formulations (e.g., fluorescent labeling) for specific applications. Lead times for recombinant protein production typically range 4-8 weeks.
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