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Citric Acid Staining Solution

Updated: 2026-07-25

Overview

Citric Acid Staining Solution is a specialized chemical formulation used primarily in the field of histology and pathology. It leverages the acidic properties of citric acid to enhance the staining of tissue samples, making cellular structures more visible under a microscope. This solution is a staple in laboratories due to its reliability and ease of use. Citric acid, the primary component, is a weak organic acid that is naturally found in citrus fruits. In staining solutions, it acts as a mordant, helping dyes bind more effectively to tissue samples. The solution is typically prepared in aqueous form and may include other additives to optimize staining performance.

Physical and Chemical Properties

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The Citric Acid Staining Solution is characterized by its clear, colorless to slightly yellow appearance. It has a density similar to water, ranging approximately between 1.0 and 1.1 g/cm³. The solution is highly soluble in water, making it easy to prepare and dilute as needed for specific staining protocols. Citric acid itself has a melting point of 153°C and decomposes at 175°C. The staining solution typically has an acidic pH, which is crucial for its role in enhancing dye uptake by tissues. Its non-toxic nature at low concentrations makes it safe for routine laboratory use, though proper handling is still recommended.

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Main Applications

The primary application of Citric Acid Staining Solution is in histology and pathology, where it is used to stain tissue samples for microscopic examination. It is particularly effective in highlighting nuclei and other cellular components, aiding in the diagnosis of diseases and research studies. Beyond medical diagnostics, this solution is also used in educational settings to teach students about tissue staining techniques. Its versatility and effectiveness make it a valuable tool in various laboratory environments, including research institutions and clinical labs.

Safety and Storage

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While Citric Acid Staining Solution is generally safe for laboratory use, it is important to follow standard safety protocols. Avoid direct contact with eyes and skin, as the acidic nature can cause irritation. Always wear gloves and protective eyewear when handling the solution. Storage conditions are straightforward: keep the solution at room temperature, away from direct sunlight and moisture. Ensure the container is tightly sealed to prevent evaporation or contamination. Proper storage will maintain the solution's efficacy over time.

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B2B Procurement Guide

When procuring Citric Acid Staining Solution, it is essential to verify the concentration and purity of the product. Different staining protocols may require specific formulations, so compatibility with existing methods should be confirmed. Purchasing from reputable suppliers ensures consistency and quality. Price ranges can vary, typically between $20 and $50 per liter, depending on the concentration and supplier. Bulk purchases may offer cost savings, but always check the shelf life and storage requirements to avoid waste. Additionally, consider the supplier's ability to provide technical support and documentation, such as Material Safety Data Sheets (MSDS).

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