Overview
Chlorophyllase is a plant-derived enzyme that cleaves the phytol tail from chlorophyll molecules, converting chlorophyll into chlorophyllide. It is widely studied for its role in plant senescence and chlorophyll degradation pathways. Industrially, it is used to modify chlorophyll for food colorants and research applications. This enzyme is typically sourced from green plants or microorganisms and is available as a lyophilized powder for laboratory and industrial use. Its activity is highly dependent on pH and temperature, requiring specific conditions for optimal performance.
Physical and Chemical Properties
Chlorophyllase is a water-soluble enzyme with a molecular structure that varies slightly depending on its source. It exhibits maximum activity at a pH range of 7-8 and temperatures around 30-40°C. The enzyme is sensitive to extreme conditions, losing activity outside these ranges. In its purified form, chlorophyllase appears as a white to light-green powder. It is stable when stored at -20°C but degrades rapidly at room temperature or in humid environments. Solubility in water is high, making it suitable for aqueous reaction systems.
Main Applications
In the food industry, chlorophyllase is used to produce chlorophyll derivatives for natural colorants, particularly in dairy and beverage products. Its ability to modify chlorophyll enhances color stability and solubility. In plant biology research, the enzyme is a key tool for studying chlorophyll metabolism and plant stress responses. It also has emerging applications in environmental science for chlorophyll detection in water quality monitoring.
Safety and Storage
While chlorophyllase is not highly toxic, direct contact with skin or eyes should be avoided. Proper PPE, including gloves and goggles, is recommended when handling the enzyme powder or solutions. Long-term storage requires freezing at -20°C in airtight containers with desiccants to prevent moisture absorption. Repeated freeze-thaw cycles should be minimized to preserve enzymatic activity.
B2B Procurement Guide
When sourcing chlorophyllase, prioritize suppliers that provide certificates of analysis (CoA) detailing enzyme activity, purity, and microbial limits. Industrial buyers should request bulk pricing for quantities above 100 grams. Consider the enzyme's source (plant vs. microbial) based on application requirements. Microbial-derived chlorophyllase may offer higher consistency for large-scale processes. Lead times can vary, so plan procurement accordingly.
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