Overview
CD9 Antibody is a monoclonal or polyclonal antibody designed to target the CD9 protein, a member of the tetraspanin family. This protein plays a crucial role in cell adhesion, motility, and signaling, making the antibody a valuable tool in biomedical research. The CD9 Antibody is extensively used in techniques such as flow cytometry, immunohistochemistry, and Western blotting to study various biological processes. The antibody is produced using recombinant DNA technology or hybridoma techniques, ensuring high specificity and affinity for the CD9 antigen. Researchers rely on this reagent for its consistency and reliability in detecting CD9 expression across different cell types and tissues.
Physical and Chemical Properties
The CD9 Antibody typically appears as a clear to slightly opaque liquid, depending on the formulation and concentration. It is soluble in aqueous buffers, making it suitable for various laboratory applications. The molecular weight of the CD9 protein targeted by the antibody is approximately 24-26 kDa, though this may vary slightly depending on the species. Storage conditions are critical to maintaining the antibody's efficacy. It should be stored at -20°C, with precautions taken to avoid repeated freeze-thaw cycles, which can degrade the antibody. Proper handling, including the use of gloves and avoiding direct contact, is recommended to ensure safety and preserve the reagent's integrity.
Main Applications
The CD9 Antibody is widely used in research areas such as cancer biology, immunology, and cell biology. It helps scientists study the role of CD9 in cell adhesion, migration, and signaling pathways. In cancer research, the antibody is used to investigate CD9's involvement in tumor progression and metastasis. Additionally, the CD9 Antibody is employed in diagnostic applications, including the detection of CD9 expression in patient samples. Techniques like flow cytometry and immunohistochemistry leverage the antibody's specificity to identify and quantify CD9-positive cells, providing insights into disease mechanisms and potential therapeutic targets.
Safety and Storage
Proper handling and storage of the CD9 Antibody are essential to ensure its longevity and effectiveness. The antibody should be stored at -20°C, with aliquots prepared to minimize freeze-thaw cycles. Exposure to light and extreme temperatures should be avoided to prevent degradation. Safety precautions include wearing gloves and protective clothing when handling the antibody. Direct contact with skin or eyes should be avoided, and the reagent should be used in a well-ventilated area. In case of accidental exposure, rinse thoroughly with water and seek medical advice if necessary. Proper disposal methods should be followed to comply with laboratory safety regulations.
B2B Procurement Guide
When procuring CD9 Antibody for B2B purposes, several factors should be considered to ensure quality and suitability for specific applications. First, verify the antibody's specificity and host species to ensure compatibility with the intended experiments. Cross-reactivity with other proteins should be minimal. Second, check the antibody's application validation, such as whether it has been tested for use in Western blotting, immunohistochemistry, or flow cytometry. Pricing can vary widely, with reference ranges typically between $200 and $500 per vial, depending on the supplier and quantity. Bulk purchases may offer cost savings, but storage capacity should be evaluated to maintain product integrity.
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