Overview
CD77, also known as globotriaosylceramide (Gb3), is a glycosphingolipid that serves as a cell surface antigen. It is recognized by specific monoclonal antibodies and plays a significant role in immunological research, particularly in studies of B-cell malignancies like Burkitt's lymphoma. CD77 is widely used as a biomarker in diagnostic applications and research laboratories. The antigen is structurally characterized by its trisaccharide head group, which interacts with specific antibodies. Its expression is often associated with certain stages of B-cell development and has been implicated in various pathological conditions, making it a valuable target for both research and clinical diagnostics.
Physical and Chemical Properties
CD77 is typically supplied as a purified monoclonal antibody in liquid solution or lyophilized powder form. The antibody binds specifically to the CD77 antigen with high affinity, making it suitable for various immunological techniques such as flow cytometry, immunohistochemistry, and ELISA. In terms of stability, CD77 antibodies should be stored under recommended conditions to maintain activity. Liquid formulations are generally stable at 2-8°C, while lyophilized powders require storage at -20°C. Reconstitution should be performed using appropriate buffers to ensure optimal performance in assays.
Main Applications
CD77 antibodies are primarily used in research settings to study B-cell biology and malignancies. They serve as important tools for identifying and characterizing CD77-positive cells in various experimental systems. In clinical diagnostics, CD77 markers help in the classification of certain lymphomas and leukemias. Beyond hematological research, CD77 has been investigated in studies of infectious diseases, as it serves as a receptor for some bacterial toxins. Its role in cell signaling and apoptosis has also made it a subject of interest in broader immunological and cancer research contexts.
Safety and Storage
When working with CD77 antibodies, standard laboratory safety precautions should be observed. This includes wearing appropriate personal protective equipment and handling materials in accordance with biosafety level guidelines. The product should not be ingested or allowed to contact skin or eyes. Proper storage is crucial for maintaining antibody integrity. Liquid formulations should be kept refrigerated at 2-8°C and protected from light. Lyophilized powders must be stored at -20°C and reconstituted following manufacturer instructions. Avoid repeated freeze-thaw cycles as they can degrade antibody performance.
B2B Procurement Guide
When procuring CD77 antibodies for research or diagnostic purposes, several factors should be considered. First, verify the antibody's specificity and validation data, including relevant applications and species reactivity. Reputable suppliers should provide certificates of analysis and technical support. Compare pricing from multiple vendors, but prioritize quality and reliability over cost alone. Consider purchasing smaller quantities initially for validation testing before committing to bulk orders. Check for availability of matching secondary antibodies and other reagents needed for your specific applications.
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