CD4 ELISA Kit

Updated: 2026-09-11

Overview

The CD4 ELISA Kit is a diagnostic tool designed for the quantitative measurement of CD4 molecules in biological samples such as serum, plasma, and cell culture supernatants. CD4 is a glycoprotein found on the surface of immune cells, including T-helper cells, and is a critical marker for immune function assessment. The kit employs enzyme-linked immunosorbent assay (ELISA) technology, which is known for its high sensitivity and specificity. This kit is widely used in research and clinical settings, particularly for monitoring immune status in HIV-infected individuals. It provides reliable and reproducible results, making it a preferred choice for laboratories and diagnostic centers. The kit typically includes pre-coated plates, standards, detection antibodies, and substrates necessary for the assay.

Physical and Chemical Properties

The CD4 ELISA Kit consists of multiple components, including a microplate pre-coated with anti-CD4 antibodies, lyophilized or liquid standards, detection antibodies conjugated with enzymes, and various buffers and substrates. The kit is designed to be stable under recommended storage conditions (2-8°C), ensuring long-term usability. The assay leverages the specificity of antibody-antigen interactions, with the enzyme-linked detection system providing a colorimetric readout proportional to the CD4 concentration in the sample. The kit's performance is characterized by high precision, with low intra- and inter-assay variability, making it suitable for both research and diagnostic applications.

Main Applications

The CD4 ELISA Kit is primarily used in immunology research and clinical diagnostics. In research, it aids in studying immune responses, T-cell function, and the role of CD4 in various diseases. Clinically, it is indispensable for monitoring CD4 counts in HIV patients, which is crucial for disease progression assessment and treatment efficacy. Additionally, the kit finds applications in vaccine development, where CD4 levels are monitored to evaluate immune responses. Its high sensitivity and specificity make it suitable for detecting low CD4 concentrations in complex biological matrices, providing valuable insights into immune system dynamics.

Safety and Storage

The CD4 ELISA Kit should be handled with standard laboratory safety precautions, including the use of gloves and protective eyewear. Some components may contain preservatives or other chemicals that could be harmful if ingested or come into contact with skin. Proper disposal of kit components is essential to prevent environmental contamination. The kit must be stored at 2-8°C and protected from light to maintain stability. Repeated freeze-thaw cycles should be avoided, as they can degrade the reagents and affect assay performance. Users should always check the expiration date and ensure that the kit is used within its specified shelf life for optimal results.

B2B Procurement Guide

When procuring CD4 ELISA Kits, buyers should consider several factors to ensure they select the right product for their needs. Key considerations include the kit's sensitivity, detection range, and compatibility with the laboratory's existing equipment. It is also important to verify the supplier's reputation and the availability of technical support. Bulk purchases may offer cost savings, but buyers should ensure that the kits have a sufficient shelf life to avoid wastage. Comparing prices and specifications from multiple suppliers can help identify the best value. Additionally, buyers should confirm that the kit meets regulatory requirements for their intended use, particularly in clinical diagnostics.