Overview
The CD34 Molecule ELISA Kit is designed for the quantitative detection of CD34, a cell surface glycoprotein that serves as a marker for hematopoietic stem cells and endothelial progenitor cells. This kit is essential in research and clinical diagnostics, particularly in studies related to stem cell biology, angiogenesis, and cancer research. The ELISA (Enzyme-Linked Immunosorbent Assay) method employed by this kit ensures high sensitivity and specificity, making it a preferred choice for laboratories. The kit typically includes pre-coated microplates, standards, detection antibodies, and substrates required for the assay. It is compatible with various biological samples, including serum, plasma, and cell culture supernatants. The CD34 Molecule ELISA Kit is widely used due to its reliability and ease of use, providing accurate and reproducible results for quantitative analysis.
Physical and Chemical Properties
The CD34 Molecule ELISA Kit components are typically supplied in lyophilized or liquid form, depending on the manufacturer. The reagents are stable when stored at recommended temperatures (usually 2-8°C) and should be protected from light and moisture to maintain efficacy. The kit's detection range and sensitivity are critical parameters, often specified by the manufacturer, and can vary slightly between different batches or brands. The assay relies on the principle of sandwich ELISA, where CD34 molecules in the sample are captured by immobilized antibodies and detected using enzyme-linked secondary antibodies. This method ensures high specificity and minimizes cross-reactivity with other proteins. The kit's performance is validated through rigorous quality control tests to ensure consistency and accuracy across different laboratories.
Main Applications
The CD34 Molecule ELISA Kit is primarily used in research and clinical settings to study hematopoietic stem cells (HSCs) and endothelial progenitor cells (EPCs). CD34 is a well-established marker for these cell types, and its quantification is crucial for understanding their role in various physiological and pathological processes, including hematopoiesis, vascular repair, and tumor angiogenesis. In clinical diagnostics, the kit aids in the assessment of stem cell mobilization and transplantation efficacy. It is also employed in cancer research to investigate the role of CD34+ cells in tumor progression and metastasis. Additionally, the kit is valuable in preclinical studies for evaluating the effects of drugs or therapies on stem cell populations. Its versatility and reliability make it a staple tool in both academic and industrial laboratories.
Safety and Storage
Proper handling and storage of the CD34 Molecule ELISA Kit are essential to ensure accurate results and user safety. The kit components should be stored at 2-8°C, and repeated freeze-thaw cycles should be avoided to prevent degradation of sensitive reagents. Users should wear appropriate personal protective equipment (PPE), such as gloves and lab coats, when handling the kit to minimize exposure to potentially hazardous chemicals. The kit may contain substances that are harmful if ingested or come into contact with skin or eyes. In case of accidental exposure, rinse thoroughly with water and seek medical attention if necessary. Disposal of used reagents should comply with local regulations for biological and chemical waste. Always refer to the manufacturer's safety data sheets (SDS) for detailed hazard information and emergency procedures.
B2B Procurement Guide
When procuring the CD34 Molecule ELISA Kit for B2B purposes, it is important to consider several factors to ensure you select the right product for your needs. First, verify the kit's specifications, including detection range, sensitivity, and compatibility with your sample types. Reputable suppliers often provide validation data and certificates of analysis to confirm product quality. Compare prices and lead times from multiple suppliers to find the best value without compromising on quality. Bulk purchases may offer cost savings, but ensure the shelf life aligns with your usage timeline. Additionally, check for technical support and after-sales services, as these can be invaluable for troubleshooting or optimizing assay conditions. Establishing a relationship with a reliable supplier can streamline future procurement processes and ensure consistent product quality.
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