Overview
The CD33 antibody is a crucial biological reagent designed to bind specifically to the CD33 antigen, a transmembrane protein expressed on myeloid cells, including monocytes, granulocytes, and some leukemic blasts. It is widely utilized in research, diagnostics, and therapeutic development, particularly in the study of acute myeloid leukemia (AML). The antibody is available in various forms, including unconjugated, fluorescently labeled, or biotinylated, to suit different experimental needs. It is produced through hybridoma technology or recombinant methods, ensuring high specificity and reproducibility.
Physical and Chemical Properties
The CD33 antibody is typically supplied as a liquid solution in PBS or as a lyophilized powder. Its molecular weight ranges between 150-170 kDa, depending on the clone and conjugation method. The reagent is stable under recommended storage conditions but may degrade if exposed to repeated freeze-thaw cycles or extreme temperatures. Solubility is high in aqueous buffers, making it suitable for dilution in standard laboratory buffers. The antibody’s binding affinity is validated through ELISA or flow cytometry, ensuring reliable performance in assays.
Main Applications
The CD33 antibody is extensively used in flow cytometry for immunophenotyping, enabling the identification and characterization of myeloid cells in blood, bone marrow, and tissue samples. It is also employed in immunohistochemistry (IHC) to detect CD33 expression in fixed tissue sections. In clinical research, the antibody plays a key role in studying AML and other myeloid disorders. Additionally, it is used in the development of targeted therapies, such as antibody-drug conjugates (ADCs) like gemtuzumab ozogamicin.
Safety and Storage
Proper handling of the CD33 antibody is essential to maintain its integrity and ensure user safety. Gloves and lab coats should be worn to prevent skin contact or inhalation. The reagent should be stored at 2-8°C for short-term use or at -20°C for long-term preservation. Avoid repeated freeze-thaw cycles, as this can degrade the antibody. If lyophilized, reconstitute with sterile distilled water or a recommended buffer. Dispose of unused material according to local biohazard regulations.
B2B Procurement Guide
When procuring CD33 antibodies, verify the clone specificity and host species (e.g., mouse, rabbit) to ensure compatibility with your experimental setup. Check for validation data, such as Western blot or flow cytometry results, provided by the supplier. Consider the conjugation type (e.g., FITC, PE, APC) based on your detection method. Bulk purchases may offer cost savings, but ensure proper storage facilities are available. Reputable suppliers like BioLegend, BD Biosciences, and Abcam are recommended for quality assurance.
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