Overview
The CD15 (Lewis X) ELISA Kit is a specialized immunoassay designed to detect and quantify the CD15 antigen, a carbohydrate moiety involved in cell adhesion and immune modulation. CD15 is expressed on neutrophils, certain carcinomas, and other cell types, making it a valuable biomarker in research and diagnostics. The kit utilizes enzyme-linked immunosorbent assay (ELISA) technology, offering high sensitivity and specificity. It is widely used in academic, clinical, and pharmaceutical settings to study immune responses, cancer biology, and inflammatory diseases. The kit typically includes a pre-coated microplate, standards, detection antibodies, and substrates. Its user-friendly format allows for high-throughput screening, making it suitable for laboratories with varying levels of expertise. The quantitative nature of the assay provides reliable data for comparative studies and longitudinal monitoring of CD15 levels in biological samples.
Physical and Chemical Properties
The CD15 ELISA Kit components are formulated for stability and performance under standard laboratory conditions. The lyophilized reagents are reconstituted with distilled water or buffer, while liquid components are ready to use. The pre-coated microplate is typically made of polystyrene, optimized for antibody binding and minimal background noise. The detection system often involves horseradish peroxidase (HRP) or alkaline phosphatase (AP) enzymes, producing a colorimetric signal proportional to CD15 concentration. Key chemical properties include the specificity of the capture and detection antibodies for the CD15 epitope, ensuring minimal cross-reactivity with other antigens. The kit's buffers are designed to maintain optimal pH and ionic strength for antigen-antibody interactions. Shelf life is generally 12-18 months when stored at 2-8°C, with strict avoidance of repeated freeze-thaw cycles to preserve reagent integrity.
Main Applications
The CD15 ELISA Kit is primarily used in biomedical research to investigate the role of CD15 in immune cell interactions, inflammation, and cancer progression. It is particularly valuable in studies of neutrophil activation, as CD15 is a marker for these cells. In clinical diagnostics, the kit can aid in the detection of CD15-expressing tumors, such as certain leukemias and carcinomas, providing insights into disease staging and therapeutic monitoring. Pharmaceutical applications include drug development and screening, where the kit helps assess the efficacy of compounds targeting CD15-related pathways. Additionally, it is used in vaccine research to evaluate immune responses involving CD15-bearing pathogens. The versatility of the assay makes it a staple in laboratories focused on immunology, oncology, and infectious diseases.
Safety and Storage
Proper handling and storage of the CD15 ELISA Kit are critical for maintaining assay performance and user safety. Reagents should be stored at 2-8°C, protected from light and moisture. Lyophilized components must be reconstituted with care to avoid contamination. The stop solution, often acidic, requires special precautions to prevent skin or eye contact. Personal protective equipment, such as gloves and lab coats, is recommended during kit use. To ensure reproducibility, avoid repeated freeze-thaw cycles of liquid reagents. Kit components should be equilibrated to room temperature before use, and the microplate must be sealed properly between steps to prevent evaporation. Disposal of kit waste should comply with local regulations for chemical and biological materials, particularly if samples contain hazardous substances.
B2B Procurement Guide
When procuring CD15 ELISA Kits in bulk for B2B purposes, several factors should be considered to ensure quality and cost-effectiveness. Verify the kit's sensitivity, dynamic range, and compatibility with your sample types. Reputable suppliers should provide certificates of analysis (CoA) for each batch, confirming performance specifications. Bulk orders often qualify for discounts, but confirm lead times and storage requirements to avoid stockpiling perishable items. Evaluate the supplier's technical support and availability of replacement reagents. For international shipments, ensure compliance with customs regulations for biological materials. Some manufacturers offer custom configurations, such as larger plate formats or alternative detection methods, which may be beneficial for high-throughput applications. Always compare multiple vendors for pricing, reliability, and post-purchase support.
Related Manufacturers
- 主营：ELISA试剂盒、PCR试剂盒、生化试剂盒、检测试剂盒、蛋白、抗体、细胞
- 主营：Elisa试剂盒、生化试剂盒、蛋白试剂盒、细胞试剂盒
- 主营：Elisa试剂盒、人elisa试剂盒、大鼠elisa试剂盒、小鼠elisa试剂盒、植物elisa试剂盒、酶联免疫试剂盒
- 主营：ELISA检测试剂盒、检测试剂盒、酶联免疫检测试剂盒、PCR试剂盒
- 主营：脂蛋白、关基因、亲环素、ELISA试剂盒、PCR试剂盒、生化试剂盒、钾通道、列抗体、ntp溶液、蛋白酶、脑蛋白、病毒抗、蛋白质、239抗体、dtt溶液、科研试剂
- 主营：elisa试剂盒、检测试剂盒、PCR试剂盒
- 主营：ELISA试剂盒、检测试剂盒、生化试剂盒、pcr检测试剂盒、细胞
- 主营：ELISA试剂盒、ELISA检测试剂盒、小鼠ELISA试剂盒、人ELISA试剂盒、大鼠ELISA试剂盒、试剂盒elisa
- 主营：CD15分子、人cx3c趋化因子
- 主营：18908-66-2、癸甲氯铵、咪唑烷酮、elisa试剂盒、二异丁腈、饲料添加剂、分散剂工业、柠檬酸三丁酯、聚乙烯吡咯烷、二甲基溴化铵
- 主营：培养箱、离心机、生物安全柜、实时荧光pcr 仪、美谷分子酶标仪、超低温冰箱、电转仪、电穿孔、二氧化碳培养箱、赛默飞荧光定量PCR、二手赛默飞培养箱、伯乐荧光定量PCR仪、罗氏实时荧光量PCR、赛默飞分光光度计、赛默飞细胞计数仪、赛默飞超低温冰箱、赛默飞高速离心机、艾本德离心机、高速冷冻离心机、冷冻离心机、奥林巴斯显微镜、伯乐电穿孔仪、落地式离心机、赛默飞质谱仪、伯乐凝胶成像仪
- 主营：ELISA试剂盒、PCR试剂盒、科研试剂
- 主营：igg抗体、糖蛋白、脂蛋白、试剂盒、人瘦素、粗纤维、猪睾酮、烯二酮、nad激酶、测试盒、牛瘦素、牛胆酸、原果胶、磷酸酶、犬肾素、含水量、人蛋白、甜菜碱、igm抗体、dna抗体、磷脂酶、大鼠超、犬叶酸、人胆酸、样受体
- 主营：ELISA试剂盒、高灵敏度试剂盒、试剂盒厂家、人ELISA试剂盒、小鼠ELISA试剂盒、大鼠ELISA试剂盒、仓鼠ELISA试剂盒、豚鼠ELISA试剂盒、鸡ELISA试剂盒、鸭ELISA试剂盒、牛ELISA试剂盒、羊ELISA试剂盒、马ELISA试剂盒、猴ELISA试剂盒、猪ELISA试剂盒、犬ELISA试剂盒、兔ELISA试剂盒、鱼ELISA试剂盒、植物ELISA试剂盒、土壤ELISA试剂盒、定性ELISA试剂盒、肿瘤标志物检测ELISA试剂盒、特种蛋白检测ELISA试剂盒、传染病检测ELISA试剂盒
- 主营：原位杂交探针定制、组织固定液、组织脱钙液、原位杂交试剂盒、TSA多重荧光染色试剂盒、特殊染色液
- 主营：试剂、细胞、酶联免疫法、elisa 试剂盒、Elisa试剂盒、进口试剂盒、国产试剂盒、ELISA检测试剂盒、酶联免疫试剂盒、酶活性检测、酶标仪检测
- 主营：PCR检测、ELISA检测、酶联免疫、Elisa试剂盒、PCR试剂盒、PCR检测试剂盒、核酸检测试剂盒、荧光定量检测试剂盒、荧光定量试剂盒、比色法检测试剂盒、生化试剂盒、比色法试剂盒、酶活性比色法检测试剂盒、酶活性检测试剂盒、微量法检测试剂盒、分光光度检测试剂盒、酶活分光光度试剂盒、ELISA试剂盒、酶联免疫试剂盒、酶联免疫检测试剂盒、细胞、抗体
- 主营：试剂盒、elisa试剂盒、pcr试剂盒、细胞
- 主营：检测方、钠尿肽、同源物、试剂盒、大鼠c-肽(、elisa 检测试剂盒、ep300检测、结合蛋白、微球蛋白、抗胰蛋白酶、淋巴瘤因子、蛋白磷酸酶、死亡激动剂、核心域蛋白1、大鼠e选择素、大鼠janus激酶、激活蛋白激酶、大鼠ⅱ型胶原、大鼠ⅳ型胶原、大鼠klotho蛋白、细胞激活因子、大鼠ⅲ型胶原、大鼠nadh脱氢酶1、大鼠ⅵ型胶原α1
- 主营：酶cγ链、α2巨球、素d合成、ELISA试剂盒、纤连蛋白、抗组蛋白、肌醇抗体、蛋白样环、敏感蛋白、牛骨钙素、蛋白重链、金属肽酶、乳球蛋白、钩端螺旋、蛋白激酶、高血糖素、钙调蛋白、小鼠α1抗、pcr检测试剂、生化检测试剂
- 主营：elisa试剂盒、生化试剂盒、pcr试剂盒、抗体、细胞
- 主营：科研试剂盒、酶免试剂盒、elisa试剂盒、生化试剂盒、进口ELISA试剂盒、PCR试剂盒、细胞、生物试剂、生化测试盒
- 主营：科研试剂盒、酶免试剂盒、elisa试剂盒、进口ELISA试剂盒、PCR试剂盒、细胞、生物试剂、生化测试盒