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Brilliant Green Indicator Solution

Updated: 2026-07-15

Overview

Brilliant Green Indicator Solution is a vital staining agent in microbiological and histological laboratories. It is widely recognized for its role in differentiating bacterial species, particularly in Gram staining procedures. The solution is valued for its stability and effectiveness in highlighting cellular structures under microscopic examination. The chemical is a synthetic dye belonging to the triarylmethane class. Its ability to selectively stain certain bacterial species makes it indispensable in diagnostic and research settings. Laboratories and industrial buyers often procure it in bulk for routine staining applications.

Physical and Chemical Properties

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Brilliant Green Indicator Solution appears as a green liquid with a molecular weight of 482.63 g/mol. It is soluble in both water and alcohol, making it versatile for various staining protocols. The solution is pH-sensitive, which can influence its staining intensity and specificity. The density of the solution is approximately 1.0 g/cm³, and it should be stored in a cool, dark place to maintain its efficacy. Proper storage conditions are crucial to prevent degradation, which could compromise its performance in laboratory applications.

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Main Applications

The primary use of Brilliant Green Indicator Solution is in microbiology for staining bacterial cells. It is a key component in Gram staining, where it helps differentiate Gram-positive and Gram-negative bacteria. The solution is also employed in histology to highlight specific tissue structures. Beyond medical and research labs, the solution finds applications in water testing and environmental monitoring. Its ability to selectively stain microorganisms makes it useful in identifying contaminants in water samples.

Safety and Storage

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Brilliant Green Indicator Solution requires careful handling to avoid skin and eye contact. It should be used in a well-ventilated area, and personal protective equipment (PPE) such as gloves and goggles is recommended. In case of accidental exposure, rinse thoroughly with water and seek medical advice if necessary. Storage conditions are critical for maintaining the solution's stability. It should be kept in a tightly closed container, away from direct sunlight and extreme temperatures. Proper labeling and segregation from incompatible chemicals are also essential safety measures.

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B2B Procurement Guide

When procuring Brilliant Green Indicator Solution, buyers should verify the concentration and purity to ensure it meets their specific requirements. It is advisable to request certificates of analysis (CoA) from suppliers to confirm the product's quality and consistency. Bulk purchases often come with cost advantages, but buyers should also consider storage capacity and shelf life. Reliable suppliers with a track record in chemical distribution are preferred to ensure timely delivery and product integrity. Price ranges can vary, so obtaining multiple quotes is recommended.

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