Overview
BR Reagent, commonly known as Bromophenol Blue, is a vital chemical used in molecular biology for staining nucleic acids during gel electrophoresis. It is valued for its ability to migrate with DNA/RNA fragments, providing visual tracking of sample movement. The reagent is widely adopted in research laboratories, diagnostic centers, and educational institutions due to its reliability and ease of use. It is often combined with other staining agents to enhance detection sensitivity.
Physical and Chemical Properties
BR Reagent appears as a dark blue to purple powder, soluble in water and organic solvents like ethanol and acetone. It exhibits stability under recommended storage conditions but may degrade when exposed to prolonged light or high temperatures. Its molecular weight is 669.96 g/mol, and it decomposes around 280°C. The reagent's solubility and stability make it suitable for various laboratory applications, particularly in gel-based assays.
Main Applications
The primary use of BR Reagent is in agarose gel electrophoresis, where it serves as a tracking dye for DNA and RNA samples. It helps researchers monitor the progress of electrophoresis and estimate fragment sizes. Beyond electrophoresis, it is also employed in protein analysis and as a pH indicator in certain biochemical assays. Its versatility makes it a staple in molecular biology laboratories.
Safety and Storage
BR Reagent should be handled with care to avoid inhalation or skin contact. Proper PPE, including gloves and goggles, is recommended. In case of exposure, rinse affected areas with plenty of water. Storage should be in a cool, dry place, ideally at 2-8°C, and protected from light. Properly sealed containers are essential to prevent moisture absorption and degradation.
B2B Procurement Guide
When procuring BR Reagent, verify the supplier's credentials and request certificates of analysis (COA) to ensure product quality. Bulk purchases may offer cost savings, but ensure proper storage facilities are available. Consider suppliers with a track record in biochemical reagents and check for compliance with industry standards. Lead times and shipping conditions (e.g., cold chain for large orders) should also be confirmed.
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