Overview
Bovine Synapsin is a neuronal phosphoprotein isolated from bovine brain tissue, primarily functioning in the regulation of synaptic vesicle pools. It belongs to the synapsin family, which plays a critical role in modulating neurotransmitter release at presynaptic terminals. This protein is widely used as a biochemical tool in neuroscience research due to its conserved function across mammalian species. In B2B contexts, Bovine Synapsin is typically supplied to research institutions, pharmaceutical companies, and diagnostic manufacturers. The protein is extracted through specialized purification protocols to maintain its native structure and biological activity, often verified through phosphorylation assays and vesicle binding tests.
Physical and Chemical Properties
As a phosphoprotein, Bovine Synapsin exhibits variable molecular weight (80-100 kDa) depending on its phosphorylation state and isoform. The protein is heat-labile and susceptible to protease degradation, requiring storage at -20°C or below in lyophilized form. It demonstrates optimal solubility in neutral pH buffers (e.g., 50 mM Tris-HCl, pH 7.4). Key functional domains include the N-terminal ATP-binding site and C-terminal vesicle-binding region. The protein's activity is modulated by calcium/calmodulin-dependent kinase II (CaMKII) phosphorylation, a property exploited in synaptic plasticity studies. Commercial preparations typically include stabilizing agents like glycerol or BSA to prevent aggregation.
Main Applications
In research settings, Bovine Synapsin serves as a standard for studying synaptic vesicle dynamics, particularly in reconstitution experiments and electron microscopy studies. It's frequently used as a positive control in Western blotting for synapsin expression analysis across species. The pharmaceutical industry utilizes this protein in drug discovery platforms targeting neurotransmitter release disorders, including epilepsy and Parkinson's disease. Diagnostic applications include antibody production for neurological disease biomarkers. Some biotech companies incorporate purified synapsin into synaptic function assay kits for high-throughput screening.
Safety and Storage
While Bovine Synapsin presents minimal toxicity, standard laboratory precautions apply: use PPE (gloves, lab coat), avoid direct contact, and prevent aerosol formation. The lyophilized form is stable for 2-3 years at -80°C when desiccated, while reconstituted solutions should be aliquoted and used within 1-2 weeks. Critical storage parameters include protection from repeated freeze-thaw cycles and microbial contamination. For laboratories handling large quantities, material safety data sheets (MSDS) should be consulted regarding specific handling procedures, though the protein is generally classified as Risk Group 1 (low individual/community risk).
B2B Procurement Guide
When sourcing Bovine Synapsin, prioritize suppliers providing detailed certificates of analysis (CoA) including purity verification (SDS-PAGE/Coomassie), endotoxin levels, and functional activity data. Research-grade batches should demonstrate >90% purity with minimal protease contamination. Bulk purchasers (≥100mg) should negotiate batch consistency guarantees and request custom characterization data if needed. Consider suppliers offering technical support for application-specific queries. Lead times for specialty preparations often range 4-8 weeks. For regulatory-sensitive applications, ensure documentation of bovine sourcing complies with TSE/BSE-free certification requirements.
Related Manufacturers
- 主营：ELISA试剂盒、试剂盒、小鼠ELISA试剂盒、牛果糖胺、IL-6 ELISA、ELISA检测试剂盒、细胞因子检测试剂盒、胰岛素ELISA、皮质醇ELISA、尿调蛋白、肽释放酶、弹性蛋白、乳糖醛酸、胰蛋白酶、价铬离子、操作步骤、大鼠蛋白、结合蛋白、实验科研、科研ELISA试剂盒
- 主营：ELISA试剂盒、ELISA检测试剂盒、小鼠ELISA试剂盒、牛突触素、人ELISA试剂盒、大鼠ELISA试剂盒、试剂盒elisa
- 主营：科研试剂盒、酶免试剂盒、elisa试剂盒、生物试剂、细胞、进口ELISI试剂盒、PCR试剂盒
- 主营：科研试剂盒、酶免试剂盒、elisa试剂盒、生物试剂、细胞、进口ELISA试剂盒、PCR试剂盒、生化测试盒
- 主营：ELISA试剂盒、PCR试剂盒、生化试剂盒、科研试剂
- 主营：酶cγ链、α2巨球、素d合成、牛骨钙素、纤连蛋白、抗组蛋白、肌醇抗体、蛋白样环、敏感蛋白、蛋白重链、金属肽酶、乳球蛋白、钩端螺旋、蛋白激酶、高血糖素、钙调蛋白、小鼠α1抗、ELISA试剂盒、pcr检测试剂、生化检测试剂
- 主营：科研试剂盒、酶免试剂盒、elisa试剂盒、生物试剂、生化试剂盒、细胞、进口ELISA试剂盒、PCR试剂盒、生化测试盒
- 主营：检测方、钠尿肽、试剂盒、人突触素、同源物、ep300检测、结合蛋白、大鼠c-肽(、微球蛋白、抗胰蛋白酶、淋巴瘤因子、蛋白磷酸酶、死亡激动剂、核心域蛋白1、大鼠e选择素、大鼠janus激酶、激活蛋白激酶、大鼠ⅱ型胶原、大鼠ⅳ型胶原、大鼠klotho蛋白、细胞激活因子、大鼠ⅲ型胶原、大鼠nadh脱氢酶1、大鼠ⅵ型胶原α1、elisa 检测试剂盒
- 主营：人突触素、ELISA试剂盒、抗体
- 主营：ELISA试剂盒、PCR试剂盒、生化试剂盒、突触素ELISA试剂盒、蛋白、抗体、细胞
- 主营：突触素、elisa试剂盒
- 主营：ELISA试剂盒、小鼠ELISA试剂盒、大鼠ELISA试剂盒、生化检测试剂盒、酶联免疫检测试剂盒、人ELISA检测试剂盒、植物检测试剂盒、兔ELISA试剂盒、猪elisa检测试剂盒、犬ELISA试剂盒、科研ELISA检测试剂盒
- 主营：白细胞介素、甲状腺素、活性氧簇、生物试剂、促甲状腺激素、孕酮、促黄体生成素、心肌肌钙蛋白、检测试剂盒、植物试剂盒、试剂盒、实验室试剂、Elisa、定量分析、定性分析、酶联免疫试剂盒、科研试剂盒、免费代测
- 主营：scl-70igg、人1α羟、试剂盒、研启生物、葡聚糖、大鼠11β、16α羟基、1iga抗体、胶原c端、2-he2elisa、1igm抗体、25二羟基、17羟孕酮、dna糖苷酶、5α还原酶、去氢血栓、人胰岛素、胶原n末端、大鼠8羟基、25羟基维生、2，5寡腺苷、大鼠25羟基、11脱氢血栓、羟基壬烯醛、三磷酸肌醇
- 主营：小鼠突触素、试剂盒、信号调节激酶、人转谷氨酰胺酶