Bovine Epithelial Cadherin

Updated: 2026-08-28

Overview

Bovine Epithelial Cadherin (E-cadherin) is a transmembrane glycoprotein belonging to the cadherin superfamily. It mediates calcium-dependent cell-cell adhesion in epithelial tissues, ensuring structural integrity and polarity. E-cadherin is encoded by the CDH1 gene and consists of extracellular cadherin repeats, a single-pass transmembrane domain, and a highly conserved cytoplasmic tail that interacts with catenins. In research, bovine E-cadherin serves as a model for studying epithelial dynamics, embryonic development, and metastasis suppression. Its loss or dysfunction is associated with epithelial-mesenchymal transition (EMT), a hallmark of cancer progression. Recombinant and native forms are commercially available for experimental use.

Physical and Chemical Properties

E-cadherin is a ~120 kDa glycoprotein with post-translational modifications, including N-linked glycosylation. Its extracellular domain contains five cadherin repeats that facilitate homophilic binding in a calcium-dependent manner. The protein’s stability is pH-sensitive, with optimal function at neutral pH (7.0-7.4). In solution, E-cadherin tends to form dimers or higher-order oligomers. Lyophilized preparations require reconstitution in sterile buffers, while liquid formulations often include glycerol or albumin to prevent aggregation. Analytical techniques like SDS-PAGE and Western blotting confirm its purity and identity.

Main Applications

Bovine E-cadherin is pivotal in studying cell adhesion mechanisms, tissue architecture, and cancer biology. Researchers use it to investigate EMT, tumor suppression, and cell signaling pathways (e.g., Wnt/β-catenin). It’s also employed in diagnostic assays to detect epithelial markers in biopsies or circulating tumor cells. In tissue engineering, E-cadherin-coated surfaces promote epithelial cell attachment and monolayer formation. Pharmaceutical studies explore its role in drug delivery systems targeting epithelial barriers. Its applications extend to veterinary medicine, given its conservation across species.

Safety and Storage

While E-cadherin is non-toxic, standard laboratory precautions (gloves, lab coats) are recommended. Avoid inhalation or contact with mucous membranes. For cell culture applications, ensure sterility by using endotoxin-free preparations (<1 EU/μg). Lyophilized protein should be stored at -20°C in a desiccated environment. Reconstituted solutions are stable for 1-2 weeks at 4°C with protease inhibitors. For long-term storage, aliquot and freeze at -80°C to minimize degradation. Avoid repeated freeze-thaw cycles to maintain activity.

B2B Procurement Guide

When sourcing bovine E-cadherin, prioritize suppliers with ISO certification and batch-specific quality control data. Key parameters include purity (≥90% by SDS-PAGE), biological activity (e.g., cell adhesion assays), and low endotoxin levels. Recombinant versions offer consistency, while native proteins may retain post-translational modifications. Request certificates of analysis (CoA) for each lot. Bulk purchases (10+ mg) may qualify for discounts. Lead times vary; recombinant forms typically require 4-6 weeks for production. Consider logistics: cold-chain shipping is essential for liquid formulations.