Blood Ammonia (NH3) ELISA Kit

Updated: 2026-08-06

Overview

The Blood Ammonia (NH3) ELISA Kit is designed for the quantitative detection of ammonia in blood samples. Ammonia is a byproduct of protein metabolism and is primarily detoxified by the liver. Elevated levels can indicate liver dysfunction or metabolic disorders such as hepatic encephalopathy and urea cycle defects. This kit utilizes enzyme-linked immunosorbent assay (ELISA) technology, offering a reliable and standardized method for ammonia measurement. The kit includes pre-coated plates, standards, controls, and detection reagents, ensuring a streamlined workflow. It is widely used in clinical laboratories, research institutions, and pharmaceutical companies to monitor patient health and evaluate therapeutic interventions. The assay is optimized for high throughput and can process multiple samples simultaneously, making it a practical choice for large-scale studies.

Physical and Chemical Properties

The Blood Ammonia (NH3) ELISA Kit typically contains lyophilized or liquid reagents that are water-soluble. The reagents are stable when stored at 2-8°C and should be protected from light to maintain efficacy. The kit's detection system relies on the enzymatic reaction of horseradish peroxidase (HRP) or similar enzymes, which produce a measurable color change proportional to the ammonia concentration. The assay demonstrates high sensitivity, with detection limits often in the micromolar range, and excellent specificity for ammonia over other nitrogenous compounds. The reagents are formulated to minimize interference from common blood components, ensuring accurate results even in complex matrices like plasma or serum.

Main Applications

The primary application of the Blood Ammonia (NH3) ELISA Kit is in clinical diagnostics, particularly for assessing liver function and detecting metabolic disorders. Elevated blood ammonia levels are associated with conditions such as hepatic encephalopathy, Reye's syndrome, and urea cycle disorders. The kit is also used in research settings to study ammonia metabolism and evaluate the efficacy of potential therapeutics. In addition to human diagnostics, the kit may be adapted for veterinary use to monitor ammonia levels in animals. Pharmaceutical companies utilize the kit during drug development to assess the impact of new compounds on ammonia metabolism. Its versatility and reliability make it a valuable tool across multiple disciplines.

Safety and Storage

The reagents in the Blood Ammonia (NH3) ELISA Kit should be handled with care, using appropriate personal protective equipment such as gloves and lab coats. Avoid ingestion, inhalation, or direct contact with skin or eyes. In case of exposure, rinse thoroughly with water and seek medical advice if necessary. The kit must be stored at 2-8°C and protected from light to maintain stability. Repeated freeze-thaw cycles should be avoided, as they may degrade the reagents. Proper storage ensures the kit's performance remains consistent throughout its shelf life, typically 6-12 months from the date of manufacture.

B2B Procurement Guide

When procuring the Blood Ammonia (NH3) ELISA Kit, consider factors such as kit compatibility with your laboratory equipment, the required sample volume, and the detection range. Verify the kit's expiration date and ensure it can be delivered and stored under appropriate conditions. Bulk purchases may qualify for discounts, so inquire about volume pricing if applicable. Reputable suppliers often provide technical support and validation data, which can be crucial for troubleshooting or assay optimization. Always request certificates of analysis to confirm the kit's performance specifications.