Overview
Biotin-Glutamine is a derivative of L-glutamine conjugated with biotin, a water-soluble vitamin. This compound is widely used in biochemical and pharmaceutical research due to its ability to bind with avidin or streptavidin with high affinity. Its applications span cell culture media supplementation, protein labeling, and bioconjugation techniques. The biotin moiety enables detection and purification processes, making it invaluable in molecular biology. Researchers frequently employ Biotin-Glutamine in studies involving cell metabolism, protein interactions, and diagnostic assays. Its stable chemical properties ensure reliable performance in various experimental conditions.
Physical and Chemical Properties
Biotin-Glutamine appears as a white to off-white crystalline powder with a molecular weight of 386.47 g/mol. It is soluble in water, dimethyl sulfoxide (DMSO), and methanol, facilitating its use in aqueous and organic solutions. The compound's stability under recommended storage conditions (−20°C, dry, and dark) ensures long-term usability. Key chemical properties include its high affinity for avidin and streptavidin, which is leveraged in pull-down assays and affinity chromatography. The presence of both biotin and glutamine allows for dual functionality, enabling researchers to study glutamine metabolism while utilizing biotin's detection capabilities.
Main Applications
Biotin-Glutamine is primarily used in cell culture to study glutamine metabolism in biotin-labeled cells. Its incorporation into media helps track cellular uptake and utilization, providing insights into metabolic pathways. The compound is also employed in bioconjugation, where it serves as a linker for attaching biotin to proteins or other biomolecules. In diagnostic applications, Biotin-Glutamine aids in developing assays for detecting specific proteins or nucleic acids. Its compatibility with streptavidin-based detection systems enhances sensitivity and specificity in techniques like ELISA, Western blotting, and flow cytometry.
Safety and Storage
Handling Biotin-Glutamine requires standard laboratory precautions. Avoid inhalation, ingestion, or direct contact with skin and eyes. Use gloves, goggles, and protective clothing when working with the compound. In case of exposure, rinse affected areas thoroughly with water and seek medical advice if necessary. Storage at −20°C in a dry, dark environment is critical to maintaining stability. Ensure the container is tightly sealed to prevent moisture absorption and degradation. Proper disposal methods should follow local regulations for chemical waste.
B2B Procurement Guide
When procuring Biotin-Glutamine, prioritize suppliers with certified quality control and reliable delivery logistics. Verify the product's purity (≥95% is recommended for most applications) and request certificates of analysis. Bulk purchases may offer cost savings, but ensure proper storage facilities are available. Consider suppliers specializing in biochemical reagents, as they often provide technical support and batch consistency. Compare prices across vendors, but balance cost with quality and service reliability. Shipping conditions should include cold chain logistics for temperature-sensitive delivery.
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