Overview
Big Endothelin-1 (Big ET-1) is a 38-amino acid precursor peptide cleaved by endothelin-converting enzymes to yield the active Endothelin-1. It plays a critical role in vascular homeostasis and is widely studied in hypertension and cardiovascular diseases. Originally isolated from endothelial cells, Big ET-1 is now synthetically produced for research. Its murine variant (小鼠大内皮素) shares high homology with human Big ET-1, making it valuable for translational studies.
Physical and Chemical Properties
As a peptide, Big ET-1 is water-soluble and stable when lyophilized. Its molecular weight (~3.8 kDa) and disulfide bonds contribute to its structural integrity. The peptide’s biological activity depends on its tertiary structure, which includes three intra-chain disulfide bridges. Degradation occurs under extreme pH or prolonged room-temperature exposure, necessitating cold storage.
Main Applications
Big ET-1 is primarily used to study endothelin system dysregulation in pulmonary hypertension, heart failure, and kidney diseases. It serves as a substrate for endothelin-converting enzyme assays. In drug development, it helps screen ET receptor antagonists. Its murine form is essential for preclinical models, particularly in genetically modified mice studies.
Safety and Storage
Though non-hazardous at research doses, Big ET-1 requires standard peptide handling protocols: use PPE, avoid direct contact, and work in ventilated areas. Lyophilized peptides should be stored at -20°C or below. Reconstituted solutions are stable for weeks at 4°C with protease inhibitors but avoid freeze-thaw cycles to prevent aggregation.
B2B Procurement Guide
Research-grade Big ET-1 is typically sold in 0.1–1 mg quantities. Key procurement criteria include HPLC purity (>95%), endotoxin levels (<1 EU/μg), and mass spectrometry validation. Reputable suppliers provide certificates of analysis (COA) and stability data. Bulk orders (10+ mg) may qualify for discounts but require validated cold shipping to maintain integrity.
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