Overview
BCO-PEG5 is a specialized linker compound combining a bicyclo[6.1.0]non-4-yne (BCO) group with a pentaethylene glycol (PEG5) spacer. The BCO group enables strain-promoted azide-alkyne cycloaddition (SPAAC), a copper-free click chemistry reaction, while the PEG5 spacer enhances solubility and reduces immunogenicity in biological systems. This dual functionality makes it invaluable for bioconjugation in drug development and biomaterial engineering. Developed as an alternative to traditional copper-catalyzed click chemistry, BCO-PEG5 is particularly useful in sensitive applications like antibody-drug conjugates (ADCs) and live-cell labeling, where copper toxicity is a concern. Its modular design allows seamless integration into complex molecular architectures.
Physical and Chemical Properties
BCO-PEG5 typically appears as a white to off-white powder or viscous liquid, depending on purity and storage conditions. It is highly soluble in water, dimethyl sulfoxide (DMSO), and polar organic solvents, facilitating its use in aqueous and organic reaction environments. The PEG5 spacer contributes to its hydrophilicity, while the BCO group remains stable under physiological conditions. The compound’s molecular weight is approximately 398.54 g/mol, with a molecular formula of C22H38O6. It exhibits excellent stability when stored at -20°C under inert gas but may degrade if exposed to moisture or prolonged room-temperature conditions. Analytical techniques like HPLC and mass spectrometry are recommended for purity verification.
Main Applications
BCO-PEG5 is widely employed in bioconjugation for drug delivery systems, particularly in antibody-drug conjugates (ADCs) and nanoparticle functionalization. Its SPAAC reactivity enables efficient coupling with azide-modified biomolecules (e.g., proteins, peptides) without cytotoxic copper catalysts, making it ideal for in vivo applications. In biomaterial science, BCO-PEG5 is used to modify hydrogels and scaffolds for controlled drug release or tissue engineering. Additionally, it serves as a crosslinker in diagnostic assays and biosensors, leveraging its biocompatibility and rapid reaction kinetics. Research labs also utilize it for fluorescent probe synthesis and live-cell imaging due to its minimal interference with biological processes.
Safety and Storage
While BCO-PEG5 is generally non-toxic, standard laboratory precautions should be followed, including the use of gloves, goggles, and fume hoods to prevent inhalation or skin contact. The compound is not classified as hazardous under GHS, but prolonged exposure should be avoided. For long-term stability, store BCO-PEG5 at -20°C in sealed, argon-flushed containers to prevent oxidation and moisture absorption. Aliquotting is recommended to minimize freeze-thaw cycles. Dispose of waste according to local regulations for organic compounds, though small quantities may be neutralized via hydrolysis under controlled conditions.
B2B Procurement Guide
When sourcing BCO-PEG5, prioritize suppliers that provide certificates of analysis (CoA) detailing purity (>95%), endotoxin levels (for biomedical use), and batch-to-batch consistency. Custom synthesis options are available for large-scale orders or modified PEG lengths. Pricing varies by quantity and purity, with bulk purchases (≥10 grams) often discounted by 20–30%. Lead times may extend to 4–6 weeks for specialized formulations. Evaluate supplier reliability through third-party reviews and technical support responsiveness, as impurities can critically impact experimental outcomes in sensitive applications like ADC development.