Overview
B-Cell Activating Factor (BAFF) is a tumor necrosis factor (TNF) superfamily member (TNFSF13B) essential for B-lymphocyte survival, proliferation, and differentiation. Discovered in 1999, it is produced primarily by myeloid cells and acts through three receptors: BAFF-R, BCMA, and TACI. BAFF exists as a homotrimer and can form virus-like 60-mer clusters for enhanced signaling. Dysregulated BAFF expression is implicated in autoimmune disorders like systemic lupus erythematosus (SLE) and rheumatoid arthritis. Therapeutic BAFF inhibitors (e.g., belimumab) are FDA-approved for SLE treatment, highlighting its clinical relevance.
Physical and Chemical Properties
BAFF is a type II transmembrane protein with a soluble ectodomain released by proteolysis. The mature soluble form comprises 152 amino acids (human) with 4 conserved cysteine residues forming disulfide bonds. Its tertiary structure features a characteristic TNF-like β-sheet sandwich fold. The protein is stable in neutral pH buffers but susceptible to denaturation at extreme pH or temperatures >60°C. Analytical methods include SDS-PAGE (31 kDa under reducing conditions), size-exclusion chromatography (trimer confirmation), and ELISA for quantification. Commercial preparations often include stabilizers like BSA or trehalose.
Main Applications
In research, recombinant BAFF is used to study B-cell development in vitro and in animal models. It is critical for long-term B-cell culture systems and hybridoma generation. Pharmaceutical applications include target validation for autoimmune therapies and biomarker studies. Diagnostically, BAFF levels are measured in serum (via ELISA) to monitor disease activity in SLE and Sjögren's syndrome. Emerging applications include CAR-T cell therapy optimization, where BAFF enhances B-cell malignancy targeting.
Safety and Storage
Recombinant BAFF requires careful handling due to its immunomodulatory effects. Use gloves and work in biosafety cabinets when reconstituting lyophilized powder. Avoid repeated freeze-thaw cycles; aliquot for long-term storage at -80°C. Carrier-free formulations minimize interference in cellular assays but may require lower storage concentrations (0.1-0.5 mg/mL) to prevent aggregation. Always verify bioactivity through NF-κB reporter assays or B-cell proliferation tests after prolonged storage.
B2B Procurement Guide
When sourcing BAFF for industrial use, prioritize vendors providing: 1) Certificate of Analysis with endotoxin levels (<0.1 EU/μg), 2) batch-specific activity data (typically EC50 ≤1 ng/mL in proliferation assays), and 3) scalability for GMP-grade needs. Bulk purchasers (e.g., pharmaceutical companies) should negotiate volume discounts (>10 mg orders) and request stability data under shipping conditions. For clinical-grade material, ensure compliance with ICH Q6B guidelines for biotherapeutic proteins.
Related Manufacturers
- 主营：ELISA试剂盒、PCR试剂盒、科研试剂
- 主营：小鼠B细胞淋巴瘤因子3、ELISA试剂盒、抗体
- 主营：基质胶、试剂盒、脑类器官、细胞株、成体干细胞、细胞培养基、重组蛋白
- 主营：科研试剂盒、酶免试剂盒、elisa试剂盒、细胞、生物试剂、进口ELISA试剂盒、PCR试剂盒、生化测试盒
- 主营：科研试剂盒、酶免试剂盒、elisa试剂盒、细胞、生化试剂盒、生物试剂、进口ELISA试剂盒、PCR试剂盒、生化测试盒
- 主营：检测方、钠尿肽、试剂盒、淋巴瘤因子、激活蛋白激酶、细胞激活因子、同源物、ep300检测、结合蛋白、大鼠c-肽(、微球蛋白、抗胰蛋白酶、蛋白磷酸酶、死亡激动剂、核心域蛋白1、大鼠e选择素、大鼠janus激酶、大鼠ⅱ型胶原、大鼠ⅳ型胶原、大鼠klotho蛋白、大鼠ⅲ型胶原、大鼠nadh脱氢酶1、大鼠ⅵ型胶原α1、elisa 检测试剂盒
- 主营：酶cγ链、α2巨球、素d合成、纤连蛋白、抗组蛋白、肌醇抗体、蛋白样环、敏感蛋白、牛骨钙素、蛋白重链、金属肽酶、乳球蛋白、钩端螺旋、蛋白激酶、高血糖素、钙调蛋白、小鼠α1抗、ELISA试剂盒、pcr检测试剂、生化检测试剂
- 主营：抑制物、糖蛋白、试剂盒、释放因子、刺激因子、生长因子、武装因子、淋巴细胞、趋化因子、活化黏附因子、凋亡抑制因子、人色氨酰、炎性蛋白、钙黏蛋白、成孔蛋白、人促睡眠肽、免疫球蛋白、节苷脂抗体、人精氨酸酶、二聚化蛋白、人β葡糖苷酶、人β内酰胺酶、肝炎病毒抗体、人潜伏膜蛋白、人二氢嘧啶酶
- 主营：生物试剂、甲状腺素、活性氧簇、白细胞介素、促甲状腺激素、孕酮、促黄体生成素、心肌肌钙蛋白、检测试剂盒、植物试剂盒、试剂盒、实验室试剂、Elisa、定量分析、定性分析、酶联免疫试剂盒、科研试剂盒、免费代测
- 主营：细胞激活因子、试剂盒
- 主营：试剂盒、ELISA试剂盒、科研ELISA试剂盒、PCR试剂盒、生化试剂盒
- 主营：细胞激活因子受体、elisa试剂盒
- 主营：ELISA试剂盒、生化检测试剂盒、PCR试剂盒、大鼠ELISA试剂盒、小鼠ELISA试剂盒、兔ELISA试剂盒、鸡ELISA试剂盒
- 主营：B细胞激活因子受体、elisa试剂盒
- 主营：细胞激活因子试剂盒、elisa试剂盒、试剂盒、抗体