Azido-PEG-OH

Updated: 2026-07-18

Overview

Azido-PEG-Hydroxyl (N3-PEG-OH) is a polyethylene glycol (PEG) derivative functionalized with an azido group at one terminus and a hydroxyl group at the other. This bifunctional design enables versatile applications in bioconjugation and material science, leveraging the 'click chemistry' reactivity of azides with alkynes or phosphines. PEG's hydrophilic nature ensures solubility in aqueous systems, while its biocompatibility makes it ideal for pharmaceutical and biomedical uses. Developed as a tool for controlled chemical modification, N3-PEG-OH bridges organic synthesis and biotechnology. Its modularity allows customization of PEG chain lengths (e.g., 1kDa, 5kDa) to tailor properties like solubility and steric effects. The compound is typically synthesized via etherification or esterification of PEG diols, followed by azide substitution.

Physical and Chemical Properties

Azido-PEG-Hydroxyl exhibits properties characteristic of PEG derivatives, including high water solubility and low immunogenicity. The azide group (-N3) absorbs at ~2100 cm⁻¹ in FTIR spectra, a key identifier. Thermal stability is moderate, with decomposition occurring above 200°C. The hydroxyl terminus (-OH) allows further derivatization via esterification or etherification. The compound's viscosity and melting point correlate with PEG chain length. Shorter chains (e.g., 1kDa) are waxy solids, while longer chains (e.g., 10kDa) may appear as viscous liquids. Storage stability is critical; azides can degrade or form hazardous byproducts (e.g., hydrazoic acid) under acidic or high-temperature conditions.

Main Applications

N3-PEG-OH is pivotal in bioconjugation, where it links biomolecules (e.g., proteins, peptides) to surfaces or other polymers via azide-alkyne cycloaddition. In drug delivery, it modifies nanoparticles or liposomes to enhance circulation time and target specificity. The hydroxyl group can anchor additional functional groups (e.g., carboxyl, amine) for further customization. In material science, this compound synthesizes hydrogels and smart polymers for tissue engineering. Its non-fouling properties reduce protein adsorption, making it valuable for medical device coatings. Emerging uses include diagnostic assays and biosensors, where PEG spacers minimize steric interference.

Safety and Storage

Azido-PEG-Hydroxyl requires careful handling due to potential azide-related hazards. Avoid contact with heavy metals or reducing agents, which may form explosive metal azides. Use personal protective equipment (PPE) and work in a fume hood when handling powders. Long-term storage demands anhydrous conditions at -20°C, preferably under argon or nitrogen. Solutions should be prepared fresh or stored with stabilizers (e.g., BHT) to prevent oxidative degradation. Dispose of waste according to local regulations for azide-containing compounds.

B2B Procurement Guide

When sourcing N3-PEG-OH, specify PEG molecular weight (e.g., 2kDa, 5kDa) and purity (typically >95% for research-grade). Technical datasheets should confirm azide functionality (e.g., via FTIR) and residual solvent levels. Bulk buyers should request customized packaging (e.g., amber vials, desiccated) to ensure stability. Suppliers often provide GMP-grade options for pharmaceutical applications. Compare lead times and logistics, as some derivatives require cold-chain shipping. Pilot batches are recommended to validate performance in end-use applications like conjugation efficiency.