Overview
Asialoorosomucoid (ASOR) is a biologically significant glycoprotein created through the enzymatic removal of terminal sialic acid residues from orosomucoid (α1-acid glycoprotein). This modification exposes galactose residues that confer specific binding affinity to the asialoglycoprotein receptor (ASGPR), which is abundantly expressed on hepatocytes. ASOR serves as a critical tool in hepatic research, particularly for studying receptor-mediated endocytosis pathways and liver-targeted drug delivery systems. The preparation of ASOR typically involves neuraminidase treatment of human or bovine orosomucoid, followed by purification processes to ensure product consistency. Its well-characterized interaction with ASGPR makes it a gold standard ligand for investigating liver-specific cellular uptake mechanisms in both basic research and pharmaceutical development contexts.
Physical and Chemical Properties
ASOR maintains the core protein structure of its parent orosomucoid molecule while exhibiting altered carbohydrate presentation. The removal of sialic acid residues reduces the protein's negative charge and increases its isoelectric point. The exposed galactose and N-acetylgalactosamine residues are responsible for the selective binding to ASGPR, with typical dissociation constants in the nanomolar range. The glycoprotein exhibits stability across a wide pH range (3-9) and maintains functionality in physiological buffers. Analytical characterization typically includes SDS-PAGE (showing a single band at ~41 kDa), lectin blotting for carbohydrate confirmation, and mass spectrometry for quality verification. Properly prepared ASOR should demonstrate <5% sialylated content when analyzed by methods like HPLC or capillary electrophoresis.
Main Applications
In biomedical research, ASOR serves three primary functions: as a specific ligand for ASGPR binding studies, as a carrier for liver-targeted drug delivery systems, and as a tool for investigating glycoprotein metabolism. Researchers frequently conjugate ASOR with fluorescent markers, radiolabels, or therapeutic payloads to track hepatic uptake and intracellular trafficking. The pharmaceutical industry utilizes ASOR-based systems for developing hepatocyte-specific delivery of antiviral drugs, gene therapies, and diagnostic agents. In basic science, ASOR helps elucidate the molecular mechanisms of receptor-mediated endocytosis and contributes to understanding liver diseases where glycoprotein metabolism is altered, such as certain forms of hepatitis and metabolic disorders.
Safety and Storage
ASOR presents minimal safety concerns when handled as a research chemical. Standard laboratory precautions including gloves and protective eyewear are recommended. The lyophilized powder is hygroscopic and requires storage at -20°C in a desiccated environment to maintain long-term stability. Reconstituted solutions should be aliquoted and stored at 4°C for short-term use (up to one week) or at -80°C for extended storage. For in vivo applications, endotoxin testing is critical as contamination can cause inflammatory responses. Sterile filtration (0.22 μm) is recommended before administration to animals. Material should be visually inspected for discoloration or particulate matter before use, as degradation products may affect experimental outcomes.
B2B Procurement Guide
When sourcing ASOR, prioritize suppliers specializing in research-grade glycoproteins. Key procurement considerations include certificate of analysis details (purity, endotoxin levels, residual enzyme activity), species of origin (human vs. bovine), and batch-to-batch consistency. Technical support for conjugation protocols can be valuable for customized applications. For bulk purchases (gram quantities), request custom synthesis documentation and consider stability testing for long-term projects. Lead times for specialty preparations may extend to 8-12 weeks. Establish quality control procedures including binding activity assays to verify functional performance upon receipt. Many suppliers offer ASOR conjugated to various markers (FITC, biotin, etc.) which may streamline research workflows despite higher per-milligram costs.
Related Manufacturers
- 主营：基质胶、试剂盒、细胞株、鼠去唾液酸糖蛋白受体、脑类器官、重组蛋白、成体干细胞、细胞培养基
- 主营：调味剂、茶味香精、食用香精、食用香料、凉茶香料、茶叶饮料、烘焙馅料、糖果点心、碳酸饮料、水溶性增香剂、红茶粉末香精、可食用茶类香精、龙井茶味香精、玫瑰味香精、可乐粉末香精、杏仁香精、雪梨香精、雪碧味香精、无花果味香精、杂果味香精、菠萝粉末香精、黄桃香精、香蕉味香精、香芋香精、白葡萄香精
- 主营：食品添加剂、饲料添加剂、营养强化剂、增稠剂、乳化剂、防腐剂、色素、磷酸盐保水剂、黄原胶、甘氨酸、酪蛋白酸钠、大豆分离蛋白、海藻酸钠、乳清蛋白粉、L-半胱氨酸、L-苯丙氨酸、L-异亮氨酸、丝氨酸、谷氨酰胺
- 主营：表皮生长因子、ELISA试剂盒、细胞因子、大鼠白介素、小鼠脂联素、重组蛋白
- 主营：植提专用酶、防腐剂系列、增稠剂系列、酶制剂、甜味剂、增稠剂、苦味剂、凉味剂、抗氧化剂、增味剂、烟熏液、营养强化剂、维生素、酸味调节剂、生物肽系列、稳定剂、提取物、氨基酸系列、保持剂、乳化剂、小分子肽、抗结剂
- 主营：ELISA试剂盒、生化检测试剂盒、酶联免疫检测试剂盒、小鼠ELISA试剂盒、大鼠ELISA试剂盒、人ELISA检测试剂盒、植物检测试剂盒、兔ELISA试剂盒、猪elisa检测试剂盒、犬ELISA试剂盒、科研ELISA检测试剂盒
- 主营：elisa试剂盒、人elisa试剂盒、植物elisa试剂盒、小鼠elisa试剂盒、大鼠elisa试剂盒、鱼elisa试剂盒、猪elisa试剂盒、牛elisa试剂盒、鸡elisa试剂盒、兔elisa试剂盒、犬elisa试剂盒
- 主营：elisa试剂盒、检测试剂盒、PCR试剂盒
- 主营：小鼠elisa试剂盒、elisa试剂盒
- 主营：elisa试剂盒、白细胞介素试剂盒、肿瘤坏死因子试剂盒、植物检测试剂盒
- 主营：试剂盒、辅激活因子、elisa试剂盒、蛋白、多肽、金标试剂盒、PCR试剂盒、抗生素、氨基酸、血清、抗体
- 主营：elisa试剂盒、生化试剂盒、PCR试剂盒、检测试剂盒、科研试剂盒
- 主营：elisa试剂盒、生化试剂盒、pcr检测试剂盒、去唾液酸糖蛋白受体1、细胞
- 主营：Elisa试剂盒、酶联免疫试剂盒、抗体、蛋白、细胞功能检测试剂盒
- 主营：igg抗体、试剂盒、脂蛋白、糖蛋白、大鼠超、人瘦素、粗纤维、猪睾酮、烯二酮、nad激酶、测试盒、牛瘦素、牛胆酸、原果胶、磷酸酶、犬肾素、含水量、人蛋白、甜菜碱、igm抗体、dna抗体、磷脂酶、犬叶酸、人胆酸、样受体
- 主营：柠檬酸镁、柠檬酸锌、柠檬酸铜、柠檬酸钙、柠檬酸氢二铵