Overview
ANA-12 is a synthetic small-molecule antagonist of the tropomyosin receptor kinase B (TrkB), the primary receptor for brain-derived neurotrophic factor (BDNF). Developed for research purposes, it selectively blocks BDNF/TrkB signaling without affecting other Trk receptors (TrkA or TrkC). This specificity makes it a critical tool in neuropharmacology and molecular neuroscience. First reported in 2012, ANA-12 has been widely adopted in studies investigating neuroplasticity, mood disorders, and neurodegenerative conditions like Alzheimer's disease. Its reversible binding mechanism allows for precise temporal control in experimental settings, distinguishing it from genetic knockout models.
Physical and Chemical Properties
ANA-12 appears as a white to off-white crystalline powder with a molecular weight of 377.36 g/mol. It demonstrates optimal solubility in dimethyl sulfoxide (DMSO) and methanol, often prepared as stock solutions for in vitro or in vivo applications. The compound exhibits stability when stored at -20°C in anhydrous conditions but degrades upon prolonged exposure to light or moisture. Its chemical structure includes a trifluoromethyl group and aromatic rings, contributing to its hydrophobic character and membrane permeability. These properties enable effective blood-brain barrier penetration in animal studies, though solubility limitations may require vehicle optimization for specific experimental protocols.
Main Applications
In neuroscience research, ANA-12 serves three primary functions: (1) Elucidating BDNF/TrkB signaling pathways in synaptic plasticity and memory formation, (2) Modeling depression-like behaviors in rodents by inhibiting neurotrophic support, and (3) Investigating therapeutic strategies for neurodegenerative diseases where BDNF dysregulation occurs. Recent studies employ ANA-12 to explore its effects on pain perception, addiction mechanisms, and neurodevelopmental disorders. Pharmaceutical researchers also use it to validate TrkB-targeting drug candidates by establishing baseline inhibition effects. Dosages typically range from 0.5-1.0 mg/kg in rodent models, though concentration must be empirically determined for each experimental system.
Safety and Storage
As a research chemical, ANA-12 requires handling with appropriate personal protective equipment (PPE) including nitrile gloves, lab coats, and eye protection. While acute toxicity data remains limited, it should be treated as potentially hazardous due to its bioactive properties. Avoid generating dust or aerosols during preparation. For long-term stability, store lyophilized powder in sealed vials with desiccant at -20°C, protected from light. Prepared solutions in DMSO remain stable for approximately 2 weeks when stored at -80°C in aliquots. Never freeze-thaw repeatedly. Dispose of waste according to institutional regulations for organic compounds.
B2B Procurement Guide
When sourcing ANA-12 for laboratory use, prioritize suppliers specializing in neuroscience research chemicals. Key procurement criteria include: (1) Certificate of Analysis confirming ≥98% purity via HPLC, (2) Batch-specific NMR and mass spectrometry reports, (3) Proper packaging with temperature-controlled shipping options. Research institutions commonly purchase 5-25mg quantities, with prices ranging from $50-$200 depending on purity and supplier reputation. Consider vendors offering custom synthesis for large-scale studies. Always verify CAS number 1092629-42-8 to avoid counterfeit products. Lead times vary from 1-4 weeks for specialty chemicals, so plan experiments accordingly.
Related Manufacturers
- 主营：加帽酶、激活剂、甘氨酸、igg抗体、稳定液、玻璃板、试剂盒、脂蛋白、超纯水、激动剂、胆固醇、盐酸盐、因质粒、扩容剂、组化笔、驴抗人、预制胶、驴抗兔、生物驴、草甘膦、重组酶、生物素、曲拉通、去除剂、封闭液
- 主营：砷酸锂、白云石、水合物、氮化锌、144205-68-5、二叔丁基、利多卡因、碳化钨钴、泛影葡胺、化合物库、1093222-27-5、羟基苯基、八钼酸铵、生化试剂、苯甲酸乙酯、熊去氧胆酸、胆酸二水合、乙酰丙酮锌、湿法云母粉、六氟磷酸盐、干法云母粉、苯碳酸甲酯、脱氧胆酸二水、氨基二琥珀酸、对乙酰氨基苯酚
- 主营：正辛胺、柠檬酸、尿嘧啶、抗皱素、绿原酸、异丁基、三辛胺、双醚芴、姜黄素、角鲨烯、精磺胺、蒜氨酸、九甘醇、氟苯基、15397-15-6、119736-16-2、4-嘧啶酮、923289-21-8、152628-02-9、苯甲酰胺、比卡鲁胺、四氢嘧啶、氨基二环、2346620-55-9、麦角硫因
- 主营：实验试剂、工具化合物、抗体抑制剂、重组蛋白
- 主营：小分子现货、科研小分子、单抗、多肽、RAS抑制剂、中间体、医药中间体、实验室用试剂、实验小分子、氯苯甲酸、咪唑乙醇、氧代丙腈、贝达喹啉、贝伐单抗、谷氨酰胺、喹啉-21氢、决明内酯、溴喹唑啉、苯基丙基、乙基己基
- 主营：385480-36、光栅尺、拉线盒、551126-12、空心轴、亨士乐、通心轴、编码器、521298-03、727221-01、635066-51、中空轴、斯堪纳、ssi接口、传感器、库伯勒、霍伯纳、heag176st、转换器、gse6-p1111、sick开关、pog9dn500i、pog9dn512i、pog9dn1000i、hog9dn2048i
- 主营：工件夹具、工业泵阀、电机减速机
- 主营：ade-35mh+、晶闸管、开关器、原装封、ab0805-t3、晶振器、aonr21307、aons62604、监视器、聚合物、ltm4643ev、微控制、加速计、放大器、定时器、传感器、nce8295ad、电子管、fdc021n30、ltm4612ev、lmh0024ma、电脑板、ltm8027iv、mpr121qr2、max489epd
- 主营：乙醛酸、铁甲酸、二甲基、羟乙基、草酰胺、3-氯丙炔、邻苯二甲、苯甲酰胺、溴氯海因、3-氯水扬酸、磷酸三甲酯、2-羟基喹喔啉、2-苯并唑啉酮、对羟基苯海因、3-环戊二羧酸、6-二氯异烟酸、2-氨基-5-氯苯酚
- 主营：变频器、触摸屏、振动传感器、接近开关
- 主营：电磁阀、开关阀、聚氨酯管、弹性缓冲器、空气过滤器、标准型气缸、齿条式摆动摆台、带过滤器减压阀、SMC、FESTO
- 主营：obm-d04pk、j7-a1i-ht、lj630-8bg、cj12-d4pk、cg40-20nk、热电偶、dtfssl-ii、fkc1810-p、hmd3-4zc1、全金属、超声波、传感器、gt3-dk55t、cg40-20nh、pra12-2ac、pra12-2ao、探测仪、qd-f-pk8t、给煤机、rcs5-240v、pra18-5ac、pra18-5ao、efa-d5ml1、控制器、开关npn