Overview
Agrin is a large proteoglycan that is crucial for the formation and maintenance of the neuromuscular junction (NMJ). It is synthesized by motor neurons and secreted into the synaptic cleft, where it induces the clustering of acetylcholine receptors (AChRs) on the postsynaptic membrane. Agrin's role in synaptic differentiation makes it a key molecule in neuroscience research. Discovered in the 1980s, agrin has since been extensively studied for its involvement in neuromuscular development and diseases such as myasthenia gravis. Its ability to modulate synaptic structures has also sparked interest in tissue engineering and regenerative medicine.
Physical and Chemical Properties
Agrin is a high-molecular-weight proteoglycan, typically ranging between 400-500 kDa. It consists of a core protein with multiple glycosaminoglycan (GAG) chains, which contribute to its solubility in aqueous solutions. The protein's structure includes laminin-like domains and epidermal growth factor (EGF)-like repeats, which are critical for its function. Agrin is stable under physiological conditions but sensitive to proteolytic degradation. It is commonly supplied as a lyophilized powder, which should be reconstituted in sterile buffers for experimental use. Its solubility in water and physiological buffers makes it suitable for in vitro and in vivo applications.
Main Applications
Agrin is primarily used in neuroscience research to study neuromuscular junction formation and maintenance. It is a key reagent in experiments investigating synaptic plasticity, AChR clustering, and the molecular mechanisms of neuromuscular diseases like myasthenia gravis and congenital myasthenic syndromes. Beyond basic research, agrin has potential applications in tissue engineering, particularly in the development of synthetic scaffolds for nerve regeneration. Its ability to promote synaptic differentiation makes it a promising candidate for therapies targeting neuromuscular disorders.
Safety and Storage
Agrin is generally non-toxic and safe to handle under standard laboratory conditions. However, as with all biological reagents, it is advisable to wear appropriate personal protective equipment (PPE), including gloves and lab coats, to minimize exposure. For long-term storage, agrin should be kept in lyophilized form at -20°C. Reconstituted solutions are stable for short-term use at 4°C but should be aliquoted to avoid repeated freeze-thaw cycles, which can degrade the protein. Always use sterile techniques to prevent contamination.
B2B Procurement Guide
When sourcing agrin for research or industrial applications, prioritize suppliers with a proven track record in protein production. Verify the product's purity (typically ≥95% by SDS-PAGE) and bioactivity through certificates of analysis (CoA). Price varies depending on purity, quantity, and supplier reputation. Bulk purchases may offer cost savings, but ensure proper storage conditions to maintain stability. Consider custom modifications (e.g., fluorescent labeling) if required for specific applications.
Related Manufacturers
- 主营：ELISA试剂盒、PCR试剂盒、生化试剂盒、蛋白、抗体、细胞、检测试剂盒
- 主营：科研试剂盒、酶免试剂盒、elisa试剂盒、生化试剂盒、细胞、生物试剂、进口ELISA试剂盒、PCR试剂盒、生化测试盒
- 主营：ELISA试剂盒、PCR试剂盒、生化试剂盒、蛋白、抗体、细胞
- 主营：试剂盒、酶联免疫检测试剂盒、检测试剂盒、人锚定蛋白、免疫球蛋白、人醛脱氢酶、磷脂酶域蛋白、人乙醇脱氢酶、寡聚化域蛋白、抗突触核蛋白、人层粘连蛋白、易位断点蛋白、人特异性蛋白、病毒样核蛋白、诱导反应蛋白、突触关联蛋白、人动力蛋白胞浆、前列腺素还原酶、人突触囊泡蛋白、溶酶体辅助蛋白、人血管抑制蛋白、磷脂酰肌醇聚糖
- 主营：Elisa试剂盒、生化试剂盒、Pcr试剂盒、细胞、抗体
- 主营：试剂盒、a4水解酶、结合蛋白、胶原抗体、抑制因子、小鼠碱脂、抗鼠抗体、生物检测、抗坏血酸、检测试纸、肌酸激酶、生长因子、同源蛋白、增加蛋白、蛋白b受体、人白三烯a4、小鼠便隐血、铁过氧化物、小鼠肉碱脂、小鼠炔雌醇、骨形成蛋白、乳酸脱氢酶、内消旋肌醇、生长抑制素、磷脂酰肌醇
- 主营：ELISA试剂盒、抗体、细胞、PCR试剂盒
- 主营：蛋白酶、蜗牛酶、心肌黄酶、亮抑肽酶、淀粉酶、乳糖酶、亮抑酶肽、硫代辅酶、细胞溶解酶、果胶酶、肌酸激酶、纤维素酶、枯草杆菌、乙醇脱氢酶、木聚糖酶、氯化溶菌酶、脂氧素、葡球菌酶、羧化辅酶、尿素酶、氯化酵素
- 主营：l谷氨酸、d谷氨酸、l亮氨酸、人补体7、人补体1q、largininehcl、人钙调素、人雌三醇、人唾液酸、人多巴胺、人强啡肽、bocl丙氨酸、l异亮氨酸、猪d二聚体、d天冬氨酸、l天冬氨酸、人白三烯c4、猪白介素12、pcdh1试剂盒、elisa试剂盒、dl半胱氨酸、人磷脂酶a2、dl谷氨酰胺、人降钙素原、人轮状病毒
- 主营：科研试剂盒、酶免试剂盒、elisa试剂盒、细胞、生物试剂、进口ELISI试剂盒、PCR试剂盒
- 主营：科研试剂盒、酶免试剂盒、elisa试剂盒、细胞、生物试剂、进口ELISA试剂盒、PCR试剂盒、生化测试盒
- 主营：ELISA试剂盒、PCR试剂盒、科研试剂
- 主营：试剂盒
- 主营：elisa试剂盒
- 主营：elisa试剂盒