Overview
ABTS Solution, chemically known as 2,2'-Azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt solution, is a widely used reagent in biochemical and pharmaceutical research. It serves as a substrate for measuring antioxidant activity due to its ability to form a stable radical cation (ABTS•+) when oxidized. This radical cation is blue-green and can be quantified spectrophotometrically, making it a valuable tool for assessing the antioxidant capacity of various compounds. ABTS Solution is particularly favored for its stability and ease of use compared to other radical-generating systems. It is commonly employed in assays to evaluate the free radical scavenging activity of natural extracts, pharmaceuticals, and food products. The solution is typically prepared in water or buffer and is sensitive to light and temperature, requiring careful storage to maintain its efficacy.
Physical and Chemical Properties
ABTS Solution appears as a blue-green liquid with a molecular weight of 548.68 g/mol. It is highly soluble in water, which facilitates its use in aqueous-based assays. The solution is stable under recommended storage conditions (2-8°C and protected from light) but can degrade if exposed to extreme temperatures or prolonged light exposure. The key chemical property of ABTS Solution is its ability to form the ABTS•+ radical upon oxidation, which absorbs light at specific wavelengths (commonly 734 nm). This property is exploited in spectrophotometric assays to measure the antioxidant activity of test samples. The reaction kinetics and stability of the radical make ABTS a reliable choice for high-throughput screening and comparative studies.
Main Applications
ABTS Solution is primarily used in biochemical and pharmaceutical research to measure antioxidant activity. It is a standard reagent in assays that evaluate the ability of compounds to scavenge free radicals, such as those found in plant extracts, dietary supplements, and pharmaceuticals. The assay is often used to compare the antioxidant potency of different samples or to monitor changes in antioxidant activity during processing or storage. In addition to research applications, ABTS Solution is employed in quality control testing for food and beverage products, where antioxidant content is a critical parameter. It is also used in environmental studies to assess the oxidative potential of pollutants and in clinical research to investigate the role of oxidative stress in disease mechanisms.
Safety and Storage
ABTS Solution should be handled with care to avoid skin and eye contact. Personal protective equipment, including gloves and safety goggles, is recommended when working with this reagent. The solution should be used in a well-ventilated area to minimize inhalation exposure. For storage, ABTS Solution must be kept at 2-8°C and protected from light to prevent degradation. Prolonged exposure to higher temperatures or direct light can reduce the reagent's effectiveness. Unused portions should be tightly sealed and returned to storage immediately after use to maintain stability.
B2B Procurement Guide
When procuring ABTS Solution for B2B purposes, it is essential to verify the purity and concentration specifications provided by the supplier. Reputable suppliers should offer certificates of analysis (CoA) detailing the solution's composition and performance characteristics. Batch-to-batch consistency is critical for reproducible results in research and testing applications. Price considerations should include the cost per unit volume and the concentration of the solution. Bulk purchases may offer cost savings, but buyers should ensure that storage capacity aligns with the product's shelf life. Additionally, evaluate supplier reliability, delivery times, and customer support to ensure a smooth procurement process.
Related Manufacturers
- 主营：ringers液、聚山梨酯、检测试剂盒、葡萄糖溶液、氯化锂溶液、dna提取试剂盒、染色液、色素
- 主营：染色液、碱性蓝、甘氨酸、dtt溶液、玉米素、氯霉素、腺嘌呤、媒介蓝、抽提液、异烟酸、试剂盒、糖原pas、酪蛋白、胆固醇、酚试剂、烟酰胺、牛磺酸、热灭活、奎尼丁、养乐多、碱性红、纤维素、皂化液、水合物、乳糖酸
- 主营：染料cy3、ifluor820、双nhs酯、即用型ABTS溶液、50x荧光、ifluor790、试剂盒、ifluor647、1mg蛋白、pcr定量、ifluor860、atp分析、橙钠盐、ifluor460、rna定量、探针hpf、派诺宁、生物素、刚果红、cy7酰肼、蛋白酶、探针apf、ifluor810、抑制剂、funxite-1、封闭剂
- 主营：检测试剂盒、Elisa试剂盒、农残检测试剂盒、即用型ABTS溶液、试剂、液相检测、测试盒、检测卡、比色法检测、荧光法检测
- 主营：即用型ABTS溶液、试剂盒、抗体、试剂
- 主营：6-二羟基吲哚、磷钼酸、艾拉莫德中间体、甲基丙二酸、光引发剂、苯芴醇、溴芬酸钠中间体、樟脑醌、钼酸钾、丝素蛋白、盐酸胍、β-丙氨酸甲酯盐酸盐、聚二羟基吲哚、7-去氢胆固醇、甘氨胆酸钠盐、半叶素、胆固醇、2-辛基十二醇、蛋白酶K、D-荧光素钾盐、光引发剂LAP、BIS-TRIS、甲基丙烯酸氧杂环丁烷
- 主营：DMF、DMAC、二丙酮醇、四甲基胍、马来酸酐、丙二醇、乙二醇、甲酸、甲醇、十二醇、柠檬酸、己二醇、甘油、四氢呋喃、碳酸丙烯酯、吐温、无水氯化钙、乙酸乙酯、氢氧化钠、二乙醇胺、司盘、过硫酸钠、氧化钙、氯化钾
- 主营：酶cγ链、α2巨球、素d合成、纤连蛋白、抗组蛋白、肌醇抗体、蛋白样环、敏感蛋白、牛骨钙素、蛋白重链、金属肽酶、乳球蛋白、钩端螺旋、蛋白激酶、高血糖素、钙调蛋白、小鼠α1抗、ELISA试剂盒、pcr检测试剂、生化检测试剂
- 主营：5-三甲基己醛、正癸酸、樊多B醛、聚乙二醇甲醚甲基丙烯酸酯、山梨醇缩水甘油醚、聚苯胺、磷脂酸铵盐、谷胱甘肽、二氧化钒、三羟甲基氨基甲烷盐酸盐、3二羟基萘、二苯酮、庚酰氯、菲尼酮、茶香醇、苹果酸、菠萝醚
- 主营：透析袋、移液器、SIGMA、赛默飞、爱思进、吸头/枪头、离心管、巴氏吸管、保存管、封板膜、细胞爬片、滤器、ELISA试剂盒
- 主营：透析袋、移液器、SIGMA、赛默飞、爱思进、康宁、离心管、巴氏吸管、保存管、封板膜、细胞爬片、滤器、ELISA试剂盒
- 主营：检测方、钠尿肽、试剂盒、同源物、ep300检测、结合蛋白、大鼠c-肽(、微球蛋白、抗胰蛋白酶、淋巴瘤因子、蛋白磷酸酶、死亡激动剂、核心域蛋白1、大鼠e选择素、大鼠janus激酶、激活蛋白激酶、大鼠ⅱ型胶原、大鼠ⅳ型胶原、大鼠klotho蛋白、细胞激活因子、大鼠ⅲ型胶原、大鼠nadh脱氢酶1、大鼠ⅵ型胶原α1、elisa 检测试剂盒
- 主营：Elisa试剂盒、生化试剂盒、蛋白试剂盒、细胞试剂盒
- 主营：中间体、尿囊素、肉豆蔻酸异丙酯、烯丙基羟乙基醚、苄基三乙基氯化铵、马来酸、乙酰苯肼、椰油基三甲基氯化铵
- 主营：吩嗪、肌氨酸钠、偶氮二异丁脒盐酸盐、V50、H酸、DDS、甲基磺酸铋、二恶唑啉、1-苯基-5-巯基四氮唑、柠檬酸铋、赛克、双氟代碳酸乙烯酯