Overview
5-Ethynyluridine is a synthetic nucleoside derivative, chemically modified with an ethynyl group at the 5-position of the uracil ring. It is primarily used in biochemical and pharmaceutical research as a building block for nucleotide analogs. The compound is of interest due to its ability to participate in click chemistry reactions, making it valuable for labeling and tracking nucleic acids. As a research chemical, 5-Ethynyluridine is not intended for therapeutic use but serves as a tool for studying RNA metabolism, nucleic acid modifications, and drug development. Its unique structure allows for selective functionalization, which is leveraged in various experimental protocols.
Physical and Chemical Properties
5-Ethynyluridine appears as a white to off-white crystalline powder with a molecular weight of 268.22 g/mol. It is soluble in water and polar organic solvents such as dimethyl sulfoxide (DMSO). The ethynyl group introduces reactivity for copper-catalyzed azide-alkyne cycloaddition (CuAAC), a widely used click chemistry reaction. The compound's stability under standard laboratory conditions makes it suitable for experimental use, though prolonged exposure to light or moisture should be avoided. Analytical techniques like HPLC and NMR are commonly employed to verify its purity and structural integrity.
Main Applications
In research, 5-Ethynyluridine is utilized to study RNA synthesis, turnover, and localization. It is incorporated into RNA molecules to enable visualization and tracking via fluorescent labeling techniques. The ethynyl group serves as a handle for attaching probes or tags, facilitating studies on gene expression and RNA-protein interactions. Pharmaceutical applications include its use as an intermediate in the development of antiviral or anticancer nucleoside analogs. Its compatibility with click chemistry expands its utility in drug discovery, particularly for targeted delivery systems and diagnostic tools.
Safety and Storage
5-Ethynyluridine should be handled in a fume hood with appropriate personal protective equipment (PPE), including gloves and lab coats. Inhalation or skin contact may cause irritation, and ingestion should be avoided. In case of exposure, rinse affected areas with water and seek medical advice. For long-term storage, the compound must be kept at -20°C in a tightly sealed container, protected from light and moisture. Stability under these conditions ensures minimal degradation over time, preserving its reactivity for experimental use.
B2B Procurement Guide
When procuring 5-Ethynyluridine, buyers should prioritize suppliers who provide certificates of analysis (CoA) detailing purity (typically ≥95%), solvent content, and spectroscopic validation (e.g., NMR, HPLC). Bulk purchases may qualify for discounts, but storage capacity must align with usage rates to prevent waste. For research institutions or pharmaceutical companies, partnering with specialized chemical suppliers ensures consistent quality and technical support. Lead times may vary, so advance planning is recommended. Pricing is influenced by purity, packaging (e.g., milligrams vs. grams), and supplier reputation.
Related Manufacturers
- 主营：单磷酸、白果酸、银杏酸、脱氧尿苷、尿苷三磷酸、澳洲茄胺、红四氮唑、白当归脑、曲氟胸苷、安石榴苷、二磷酸葡、白术内酯、四氮唑蓝、澳洲茄碱、巴卡亭iii、白藜芦醇、澳洲茄边、白当归素、脱氧腺苷、腺苷一水合、二磷酸钠盐、白花前胡丙、羟甲香豆素、菝葜皂苷元、磷酸二钠盐
- 主营：甘氨酸、溴化物、戊酸鈉、乙氧基、羟乙基、蒽甲酸、联吡啶、紫苏葶、锂电池、牛磺酸、褪黑素、磷酸铟、新癸酸、赖氨酸、溴化鏻、藏花醛、豆甾醇、维素酶、庚二酸、呋虫胺、丹参素、萘甲醛、槐豆胶、黄柏酮、元明粉
- 主营：催化剂、peg试剂、活性脂、cy5四氮、2406-34-0、乙氧基、cb-cyclam、dbco-acid、溴苯基、3694-51-7、2001-96-9、苯二胺、试剂cas、环辛烯、生物素、实验室、半乳糖、交联剂、环己炔、荧光素、ac4galnal、葡萄糖、环辛炔、直发cas、糖化学
- 主营：反应活、hydrazide、修饰剂、158757、催化剂、糖蛋白、连接子、激动剂、聚合物、活性脂、胆固醇、钆特酸、催化性、dna标记、络合剂、荧光试剂、雨蛙素、乙二胺、ped结构、衍生物、化合物、链接剂、反应物、生物素
- 主营：化学试剂、科研试剂、实验室试剂、分析试剂、有机试剂、生物试剂
- 主营：甘氨酸、阻燃剂、胶黏剂、3282-24-4、乙氧基、2768-02-7、7377-08-4、4865-84-3、5471-63-6、甲基橙、4355-11-7、蒽甲酸、退除剂、中性红、肉桂醛、退镍液、增弹剂、环氧胶、阻燃毯、亮氨酸、抛光盐、戊二酸、插入管、防水剂、成核剂
- 主营：95740、荧光染料、花青素染料
- 主营：溶蛋白、胰蛋白酶、玉米醇蛋白、蔗糖、硫代半乳糖苷、TRIS
- 主营：网站建设、企业官网设计、网站搭建
- 主营：乙炔基、白蜡树素、百两金素、半齿泽兰素
- 主营：异硫氰酸胍、二硫苏糖醇(DTT)、蛋白酶k、三(羟甲基)氨基甲烷tris、β巯基乙醇、十二烷基苯磺酸钠、焦碳酸二乙酯DEPC、EDTA二钠
- 主营：17746-77-9、625392-06-5、三癸基铝、1423716-55-5、1547041-34-8、甲氧苯基、8-二羟基萘、三十二烷基铝、甲氧基苯甲酸、4-氯嘧啶盐酸盐、磺胺曲沙唑
- 主营：荧光染料、荧光探针、转染试剂、试剂盒、胎牛血清、抽提试剂、萤光素钠盐、萤光素钾盐、核酸荧光染料、抗荧光淬灭剂
- 主营：酒石酸、沃尼妙林、丹诺沙星、1689-89-0、61438-64-0、81810-66-4、17316-67-5、31430-15-6、68786-66-3、42461-84-7、115550-35-1、217500-96-4、101831-37-2、129496-10-2、非泼罗尼、加米霉素、能霉素钠、达氟沙星、奥苯达唑、粘杆菌素、双氢链霉素、酸泰妙菌素、盐酸四咪唑、盐酸沙拉沙星
- 主营：咽鳞癌细胞、结肠癌细胞、急性淋巴细胞、乙炔基、永生化表皮细胞