Overview
4-Hydroxyproline (Hyp) is a non-essential amino acid derivative predominantly found in collagen, the most abundant protein in mammals. It is synthesized post-translationally by the hydroxylation of proline residues, a process critical for collagen's triple-helix stability. Due to its unique structural role, Hyp is widely studied in biochemistry and medicine. Unlike standard amino acids, Hyp is not directly incorporated into proteins during translation. Instead, it forms after proline is integrated into collagen chains, making it a marker for collagen turnover. This property is leveraged in clinical diagnostics and research.
Physical and Chemical Properties
4-Hydroxyproline is a white crystalline solid with a molecular weight of 131.13 g/mol. It decomposes at around 274°C and is highly soluble in water but only sparingly in organic solvents like ethanol. Its chiral center at the 4-position gives it optical activity, typically as the L-enantiomer in biological systems. The hydroxyl group at the 4-position enhances hydrogen bonding, contributing to collagen's thermal stability. This property is exploited in industrial applications where temperature resistance is required. Analytical techniques like HPLC and mass spectrometry are commonly used to quantify Hyp due to its specificity in collagen matrices.
Main Applications
In pharmaceuticals, 4-Hydroxyproline is used in wound healing formulations and as a precursor for synthesizing collagen-based biomaterials. Its role in skin health has also made it a popular ingredient in anti-aging cosmetics and dietary supplements targeting joint and connective tissue support. Research applications include studying fibrosis and connective tissue disorders, where Hyp levels serve as biomarkers. Industrially, it is used in gelatin production and as a stabilizer in food additives. Recent advances explore its potential in regenerative medicine, such as 3D-printed tissue scaffolds.
Safety and Storage
4-Hydroxyproline is generally regarded as safe (GRAS) for its intended uses. However, prolonged exposure to dust may irritate the respiratory tract, necessitating PPE like masks and gloves in industrial settings. It should be stored in airtight containers at room temperature, protected from humidity and light to prevent degradation. Regulatory compliance varies by application; pharmaceutical-grade Hyp requires adherence to USP or EP standards. Disposal should follow local guidelines for organic compounds. Bulk handling demands attention to electrostatic charges, as the powdered form can be combustible under specific conditions.
B2B Procurement Guide
When sourcing 4-Hydroxyproline, prioritize suppliers with ISO or GMP certifications to ensure quality consistency. Key specifications include purity (≥98%), enantiomeric purity (L-form), and absence of heavy metals. Request COAs (Certificates of Analysis) and batch-specific testing data. Pricing fluctuates based on purity and order volume, with bulk purchases (25kg+) often discounted. Lead times vary; secure agreements with multiple suppliers to mitigate supply chain risks. For research-grade Hyp, consider specialized vendors offering analytical standards with traceable documentation.
Related Manufacturers
- 主营：脯氨酸甲酯盐酸盐、聚苯胺、苄基三甲基氯化铵
- 主营：聚乙烯亚胺、三甘醇二异辛酸酯3G8、TS-1钛硅分子筛、三硫代碳酸钠40%、六氯环三磷腈、5-甲基靛红、烷基苯磺酸酯、苯基次磷酸钠、甲巯咪唑、吡啶硫酮锌、焦磷酸哌嗪、热敏材料、油酸甲酯、曲酸
- 主营：石油醚、四氢噻吩、顺酐、4α、苯酐
- 主营：化学试剂、氯金酸、卡尔费休、羟基脯氨酸、乙醇、异丙醇、药用辅料、依地酸钙钠、碳酸钠、盐酸氨丁三醇、龙胆酸、枸橼酸钠、甲醇、氢氧化钠、钴锰催化剂、硫酸锰、乙腈、色谱试剂、助剂、指示剂、醋酸锰、醋酸钴、氨丁三醇、尿素、硼酸
- 主营：环戊酮、甲酸镍、阻燃剂、羟脯氨酸、草酸镧、钼酸铵、催化剂、钒酸铋、溴氨酸、硝酸锆、碳酸镧、白僵菌、磷酸铁、钨酸酐、氟化镧、视黄醇、叔丁醇、杀螨剂、杀虫剂、醋酸钬、抗粘剂、氟化镥、肟菌酯、氢化钠、氯铂酸、氟化镁
- 主营：甜菊糖、甘氨酸、水苏糖、脯氨酸、海藻糖、辣椒精、儿茶素、甘露醇、弹脆素、酪蛋白、褪黑素、植物胶、温轮胶、赖氨酸、卡拉胶、樱桃红、薄荷冰、黄原胶、魔芋粉、海藻酸钠、精氨酸、木糖醇、瓜尔胶、胍基乙酸、维生素C
- 主营：磷钼酸、艾拉莫德中间体、甲基丙二酸、6-二羟基吲哚、聚二羟基吲哚、光引发剂、苯芴醇、溴芬酸钠中间体、樟脑醌、钼酸钾、丝素蛋白、盐酸胍、β-丙氨酸甲酯盐酸盐、7-去氢胆固醇、甘氨胆酸钠盐、半叶素、胆固醇、2-辛基十二醇、蛋白酶K、D-荧光素钾盐、光引发剂LAP、BIS-TRIS、甲基丙烯酸氧杂环丁烷
- 主营：甲基丙烯酸十二酯、甲基丙烯酸十六酯、甲基丙烯酸十八酯、羟基脯氨酸、丙烯酸十二酯、丙烯酸十六酯、丙烯酸十八酯、V50引发剂、偶氮二异丁咪唑啉盐酸盐、N-乙酰己内酰胺、硅烷偶联剂、双马来酰亚胺、马来酸酯、缩水甘油酯、缩水甘油醚、甲基咪唑、N-异丙基丙烯酰胺、金属萃取剂
- 主营：离子液体、5-二甲基吡唑、咪唑、羟基脯氨酸、催化剂、阻燃剂、正丁醇锆、四氟硼酸锂、香兰基丁醚、氟化物、全氟辛基三甲氧基硅烷、丙酮肟、双酚E型氰酸酯、叔丁基乙烯基醚、钯碳催化剂、钛硅分子筛、硫酸烟酰苯胺、丙酮缩氨基硫脲
- 主营：化学品、香精香料、试剂、羟基脯氨酸、中间体、有机硅、橡胶树脂、催化剂、纳米材料
- 主营：10ul吸头、巴氏吸管、离心管、H5534、透析袋、琼脂糖、国产试剂
- 主营：羟基脯氨酸厂家、低聚果糖
- 主营：叔碳酸、十八醛、溶菌酶、羟基癸酸、10-羟基羊蜡酸、海藻糖、癸二醇、氨基丙基、氢路噻嗪、邻苯二甲、乙基碳二、正十七烷、谷胱甘肽、烷二甲醇、松果体素、vc磷酸酯镁、2-甲基丁醛、朱栾倍半萜、丁烷四乙酸、表面活性剂、癸烷撑二醇、缩水甘油邻、氢氯噻嗪杂质、盐酸邻菲罗啉、壳聚糖季铵盐
- 主营：羟基脯氨酸、高铁酸钾
- 主营：海藻酸钠、黄原胶、酪蛋白酸钠、羟基脯氨酸、卡拉胶、胍基乙酸、瓜尔胶/瓜尔豆胶、核苷酸、L-精氨酸、亚硫酸氢钠、苹果酸、柠檬酸、乳酸链球菌素、明胶、木糖、低聚果糖、苏氨酸、色氨酸、牛磺酸、山梨酸钾、L-半胱氨酸盐酸盐、β-胡萝卜素