Overview
The 42°C Growth Medium is a specialized microbiological formulation designed for the selective cultivation of microorganisms capable of thriving at elevated temperatures. This medium serves as a critical tool in environmental monitoring and quality control processes where thermotolerant organisms serve as indicators of contamination. The development of this medium stems from the need to selectively grow heat-resistant bacteria while suppressing mesophilic organisms. Its formulation typically includes a carbon source, nitrogen compounds, minerals, and often selective agents like bile salts or antibiotics. The 42°C incubation temperature serves as both a selective and differential parameter in microbial identification protocols.
Physical and Chemical Properties
In its dry form, the medium appears as a homogeneous powder with slight color variations depending on the specific formulation. When properly prepared, it yields a clear to slightly opaque solution with a pH typically ranging from 7.2 to 7.4, optimal for most target organisms. The medium's thermal stability is a key characteristic, maintaining its structural integrity and selective properties when incubated at 42°C for 24-48 hours. Most formulations include pH indicators that change color in response to microbial metabolism, providing visual confirmation of growth. The osmotic pressure and redox potential are carefully balanced to support the target organisms while creating unfavorable conditions for non-target species.
Main Applications
This medium is primarily employed in water quality testing for the detection of thermotolerant coliforms, particularly in compliance with international standards for drinking water safety. Regulatory bodies worldwide recognize its use as a reliable indicator of fecal contamination. In clinical microbiology, the medium aids in isolating and identifying pathogens from specimens that may contain mixed flora. The food industry utilizes it for hygiene monitoring in production environments and finished products. Recent applications have expanded to include microbial ecology studies of thermophilic environments and quality control in pharmaceutical manufacturing processes where heat-resistant contaminants are a concern.
Safety and Storage
While generally non-toxic, proper handling procedures should be followed as with all microbiological media. Prepared media should be used within their specified shelf life, typically 1-4 weeks when stored refrigerated, to ensure optimal performance. Dry powder formulations have a longer shelf life, usually 1-2 years when stored in sealed containers at 2-8°C, protected from moisture and light. Once prepared, the medium should be sterilized according to manufacturer instructions, commonly through autoclaving at 121°C for 15 minutes. Quality control testing with reference strains is recommended for each new batch to verify performance characteristics.
B2B Procurement Guide
When sourcing 42°C Growth Medium, buyers should first verify the medium meets relevant regulatory standards for their intended application (e.g., ISO, USP, or EP standards). Volume discounts are typically available for bulk purchases of powdered formulations. Technical specifications to evaluate include the medium's selectivity index, growth promotion properties, and batch-to-batch consistency. Consider suppliers who provide certificates of analysis and performance testing data. For laboratories with high throughput needs, pre-poured plates or ready-to-use liquid formulations may offer significant time savings despite higher unit costs. Lead times can vary from 1-4 weeks depending on formulation complexity and customization requirements.
Related Manufacturers
- 主营：试剂盒、增菌液、平板9cm、培养基、m17琼脂、化妆品、blb琼脂、培养皿、肠球菌、mrs琼脂、gam琼脂、bhi琼脂、肉汤lb2、厌氧产、营养琼脂、菌落计数、采样管、采样袋、水样采集袋、菌片、分离培养、琼脂平板、食品检验、药典、鉴定条
- 主营：培养基、化工
- 主营：病毒采样管、DNA聚合酶、清洁验证棉签、细胞培养基、无菌拭子、粪便采样器、粪便保存液、唾液采集器、CHG涂药器、TOC瓶、血样采集卡
- 主营：高纯度原料、完全培养基、微生物、昆虫细胞、肿瘤成纤维细胞
- 主营：819刀片、PCR管、微孔板、梯度降温冻存盒、内皮细胞培养基ECM、细胞板、蛋白酶、八联管、转印膜、病理刀片、免疫组化笔、细胞计数板、离心管、降温盒、移液槽、蛋白定量试剂盒、病理切片石蜡、PBS片剂、ECL发光液、二甲基亚砜、PCR封板膜、PVDF转印膜、盒装吸头、湿转印仪、牛血清白蛋白
- 主营：一恒培养箱、生物安全柜、米欧实验室小仪器、奥浦迈培养基、宝予德移液器、弗鲁克电动搅拌器
- 主营：培养皿、菌检测、菌落计数器、培养基、ms培养基、支原体培养基、路桥培养基、马铃薯葡萄糖琼脂培养基、三药培养基、海博培养基、水质检测、微生物检测仪器设备、接种环、微生物检验、微生物检测、程控定量封口机、菌种鉴定
- 主营：硫乙醇酸盐流体对照培、沙氏葡萄糖琼脂对照培、胰酪大豆胨琼脂对照培、R2A琼脂对照培养基、R2A琼脂培养基、TCBS琼脂培养基、大豆酪蛋白琼脂培养基、麦康凯液体培养基、孟加拉红培养基、平板计数琼脂培养基、沙氏葡萄糖琼脂培养基、胰酪大豆胨琼脂培养基、胰酪大豆胨液体培养基、营养琼脂培养基、胰酪大豆胨液体对照培、甘露醇氯化钠琼脂、结晶紫中性红胆盐琼脂、湛江博康鲎试剂
- 主营：原代细胞培养、原代细胞、原代细胞冻存、细胞培养基双抗、调控细胞培养基、活性细胞培养基、生物生长培育液、活性细胞培养基成分、优化细胞培养基PH、样本生长养护原液、区分细胞培养基种类、全面讲解细胞培养基、试样增殖滋养料、离体焕活滋养源、组织增殖养护液、组织繁育养护基、生物修护养护液、样本繁育养护溶剂、活体培育营养底料、分子实验研究、原代细胞转染、试样繁育培植液、生物培植养分液、原代细胞提取定制、离体修护培植料
- 主营：诱导培养基、原代细胞、培养箱、染色试剂盒
- 主营：染色液、包埋盒、存储盒、增菌培养基、病理耗材、生理盐水、中性树胶、染色试剂、荧光染色、女性拭子、实验室耗材、组织固定液、唾液采集器、磨砂玻璃片、羽毛牌刀片、载玻片染色架、载玻片晾片板、荧光检测耗材、一次性采样管、医疗废物包装袋、全自动核酸提取、宫颈细胞保存液、不锈钢包埋底模、玻璃搅拌子、玻璃圆底蒸发皿
- 主营：cbn砂轮、etc系统、喷涂机、生长培养基、压缩机、过滤机、cpp薄膜、处理器、din导轨、led点胶、ac-600-ec、hay合并、cpu风扇、gige相机、adme毒物、ncov测定、dj控制器、pvd涂层机、端铣刀座、api连接器、led加热器、led楼梯灯、hsc起重机、电源模块、ccd摄像机、led固化灯
- 主营：吩嗪、肌氨酸钠、偶氮二异丁脒盐酸盐、培养基、V50、H酸、DDS、甲基磺酸铋、二恶唑啉、1-苯基-5-巯基四氮唑、柠檬酸铋、赛克、双氟代碳酸乙烯酯
- 主营：培养基、科研试剂、高效试剂
- 主营：加帽酶、激活剂、甘氨酸、类器官生长培养基、igg抗体、稳定液、玻璃板、试剂盒、脂蛋白、超纯水、激动剂、胆固醇、盐酸盐、因质粒、扩容剂、组化笔、驴抗人、预制胶、驴抗兔、生物驴、草甘膦、重组酶、生物素、曲拉通、去除剂、封闭液