2-Thiouridine

Updated: 2026-09-11

Overview

2-Thiouridine is a sulfur-modified nucleoside derived from uridine, where the oxygen atom at the 2-position is replaced by sulfur. This modification significantly alters the chemical and physical properties of the nucleoside, making it a valuable tool in biochemical and pharmaceutical research. It is naturally occurring in some tRNA molecules, where it plays a role in stabilizing the RNA structure and improving the accuracy of codon-anticodon interactions. The presence of sulfur enhances the rigidity of the RNA backbone, which is crucial for proper function in translation.

Physical and Chemical Properties

2-Thiouridine is a white to off-white powder with a molecular weight of 260.27 g/mol. It is soluble in water and polar organic solvents, making it suitable for various laboratory applications. The sulfur substitution at the 2-position increases its UV absorbance, which is useful for spectroscopic studies. The compound is sensitive to light and moisture, requiring storage at -20°C in a dry, dark environment. Its stability under different pH conditions and temperatures is an area of ongoing research, particularly for applications in RNA therapeutics and diagnostics.

Main Applications

2-Thiouridine is primarily used in RNA research, where it serves as a model compound to study the effects of sulfur modifications on RNA structure and function. It is also employed in the development of RNA-based therapeutics, where modified nucleosides can enhance stability and efficacy. In addition, 2-thiouridine is used as a reference standard in analytical chemistry, particularly in HPLC and mass spectrometry. Its unique properties make it a valuable tool for quality control in the production of nucleic acid-based drugs and diagnostics.

Safety and Storage

Proper handling and storage of 2-thiouridine are essential to maintain its integrity and prevent degradation. The compound should be stored at -20°C in a tightly sealed container, protected from light and moisture. Exposure to high temperatures or humidity can lead to decomposition. Safety precautions include wearing appropriate personal protective equipment (PPE), such as gloves and lab coats, to avoid skin contact and inhalation. In case of accidental exposure, rinse thoroughly with water and seek medical advice if necessary.

B2B Procurement Guide

When procuring 2-thiouridine, it is important to verify the purity and specifications provided by the supplier. High-purity grades are essential for research and pharmaceutical applications. Bulk quantities may be available at discounted rates, but proper storage facilities must be ensured upon delivery. Procurement professionals should also consider the supplier's reputation, lead times, and compliance with regulatory standards. Custom synthesis options may be available for specialized applications, but these typically require longer lead times and higher costs.