Overview
13-Cell Complete Medium is a complex nutritional solution formulated to support the growth of diverse cell types in laboratory settings. It represents an advancement over basic media by incorporating a balanced mix of essential components required by multiple cell lineages. The '13' designation typically refers to its suitability for 13 common cell types, though formulations may vary by manufacturer. This medium is particularly valuable in research and industrial applications where maintaining multiple cell lines simultaneously is necessary. Its standardized composition reduces batch-to-bariability, a critical factor in reproducible scientific experiments and biomanufacturing processes. The medium is typically supplied as a sterile liquid, ready for use after supplementation with specific growth factors or serum as required by the application.
Physical and Chemical Properties
The physical appearance of 13-Cell Complete Medium is typically clear with possible slight opalescence, often colored by pH indicators like phenol red. The pH is carefully buffered (usually 7.2-7.4) to maintain physiological conditions. Osmolarity ranges between 280-320 mOsm/kg to match intracellular conditions. Chemically, the medium contains a balanced salt solution as its base, supplemented with essential amino acids (both essential and non-essential), vitamins, trace elements, and energy sources like glucose. Many formulations also include organic compounds such as HEPES buffer for improved pH stability and sodium pyruvate as an additional energy source. The exact composition is proprietary information for most manufacturers, with variations designed to optimize performance for specific cell types or applications.
Main Applications
13-Cell Complete Medium finds extensive use in biomedical research, particularly in studies requiring simultaneous culture of multiple cell types. It's commonly employed in cancer research, stem cell studies, and virology research where different cell lines are used as hosts or controls. The medium's versatility makes it valuable for establishing co-culture systems that mimic in vivo microenvironments. In industrial applications, this medium is used in biopharmaceutical production for monoclonal antibody development and vaccine manufacturing. Its standardized formulation helps maintain consistency across production batches. Clinical applications include cell therapy production and diagnostic cell culture procedures. The medium's ability to support diverse cell types reduces the need for multiple specialized media, streamlining laboratory workflows and reducing costs.
Safety and Storage
Proper handling of 13-Cell Complete Medium requires adherence to biosafety level 2 (BSL-2) practices when working with potentially hazardous cell lines. The medium itself is generally non-hazardous, but may support microbial growth if contaminated. Always inspect for cloudiness or color changes that might indicate contamination before use. Storage at 2-8°C is recommended for short-term use (typically 1-2 months), while aliquots for long-term storage should be kept at -20°C to preserve stability. Avoid repeated freeze-thaw cycles as this can degrade heat-sensitive components. The medium should be equilibrated to 37°C before use, but prolonged warming should be avoided to prevent degradation of nutrients. Always use sterile techniques when handling to maintain aseptic conditions.
B2B Procurement Guide
When procuring 13-Cell Complete Medium in bulk for B2B applications, several factors should be considered. First, evaluate the manufacturer's quality control processes and their ability to provide consistent batches. Request certificates of analysis for important parameters like endotoxin levels (should be <1 EU/mL), sterility testing, and performance testing with relevant cell lines. Consider volume discounts and delivery schedules - many suppliers offer contract manufacturing arrangements for large-scale users. For specialized applications, inquire about custom formulation options. Compare shipping and storage requirements, as some suppliers offer cold chain logistics services. Establish quality agreements that specify acceptance criteria and protocols for handling out-of-specification products. Lead times can vary significantly (typically 2-8 weeks), so plan procurement accordingly to avoid stockouts.
Related Manufacturers
- 主营：肾细胞、培养基、周细胞、昆虫细胞、专业细胞、淋巴细胞、原代细胞、组织细胞、星状细胞、内皮细胞、成骨细胞、上皮细胞、骨样细胞、胰岛β细胞、神经元细胞、垂体瘤细胞、畸胎瘤细胞、杂交瘤细胞、乳腺癌细胞、仓鼠肺细胞、小梁网细胞、平滑肌细胞、胰岛瘤细胞、胶质瘤细胞、视网膜
- 主营：磷酸三甲苯酯、氨基甲酸乙酯、磷酸甲苯二苯酯、微生物培养基、甲乙酮肟、四乙基溴化铵、碳酸二丁酯、环烷酸铅、亚硫酸铵、二苯甲酮腙、石油磺酸钠、三乙醇胺、甲醇、乙醇、二甲苯、二氯甲烷、环烷酸铜、氨基甲酸甲酯、单过硫酸氢钾、氯化石蜡、三异丙醇胺、苄基三甲基氯化铵、苄基三乙基氯化铵、四乙基氯化铵、四甲基氯化铵、新癸酸铋
- 主营：蛋白胨、多聚甲醛、螯合铁、微生物培养基、黄血盐钠、三氯乙烯、戊二醛、对苯二胺、异辛醇、甲醇、蛋氨酸、甲醇钠、乙氧基喹啉、硼酸、酵母浸膏、磷酸二氢钾、溶剂油、磷酸、苯酚、磁黄铁矿、正丁醇、黄丹粉、二甲基甲酰胺、四氢呋喃、甲基叔丁基醚
- 主营：七水硫酸锌、七水硫酸亚铁、一水硫酸锌、培养基、一水硫酸锰、一水硫酸亚铁、五水硫酸铜、工业级七水硫酸亚铁、活性氧化锌、碱式碳酸锌、一水硫酸锌颗粒、工业七水硫酸锌、国标七水硫酸锌、农用七水硫酸锌、食品级七水硫酸锌、硫酸亚铁、农用一水硫酸锰、工业级一水硫酸锰
- 主营：大鼠白介素、小鼠脂联素、表皮生长因子、细胞因子、ELISA试剂盒、重组蛋白
- 主营：培养基、组织芯片
- 主营：杀菌剂、椰子油、印染剂、培养基、阻燃剂、油酸锌、润湿剂、硫酸铯、催化剂、粘合剂、乳化剂、草酸锰、硫酸锂、石英砂、绝缘油、茶皂素、防腐剂、醋酸镁、锂电池、碘化锂、蒸馏水、硬脂酸、塑化剂、草酸钾、磷酸铁
- 主营：蛋白胨、酪蛋白胨、大豆蛋白胨、培养基、酵母浸粉、胰蛋白胨、酸水解酪蛋白胨、牛肉浸粉、酵母浸膏、牛肉浸膏、胰酪蛋白胨、玉米浆干粉、牛肉蛋白胨、马铃薯浸粉、酵母蛋白胨、鱼蛋白胨、麦芽浸粉、消化血清粉、月示蛋白胨、牛骨蛋白胨、豆粕提取物、琼脂粉、胃蛋白胨、多价胨、多聚蛋白胨、黄豆饼粉
- 主营：程控定量封口机、97孔定量盘、酶底物法试剂、酶底物法培养基、CST测试仪、醋酸铅纸带、润滑油检测仪、自动漩涡混合器、澄明度检测仪、细菌内毒素测定仪、防爆温湿度计、电线电缆长度测量仪、地毯平毛机、高压注脂枪、外夹式通球指示器、电阻率自动测定仪、深水温度计、暗箱式紫外分析仪、红外烘烤灯、颗粒强度仪、可控硅移相调节器、等强度粱实验装置、程控静态电阻应变仪、电测水位计、激光功率计、不锈钢贝勒管
- 主营：氢氧化钠、次氯酸钠、小苏打、培养基、纯碱、聚合氯化铝、除磷剂、葡萄糖、柠檬酸、工业盐、融雪剂、聚丙烯酰胺、磷酸三钠、硼砂、液碱、食品片碱、粒碱、碳酸氢铵、高效除磷剂、聚合硫酸铁、工业除磷剂、工业级国标除磷剂、工业级聚合硫酸铁、污水处理除磷剂、净水絮凝剂、絮凝沉淀脱色剂
- 主营：啫喱胶、火柴胶、工业胶花、培养基、装订边胶、骨胶工业、工业片状胶、颗粒胶、工业纱布胶、抛光工艺明胶、冶炼工业明胶、果冻胶、工业明胶、工业蛋白粉、牛胶片、牛骨胶
- 主营：甲醇、乙醇、PVP、培养基、航空煤油、界面剂、溶剂油、苯甲醇、偶氮二异丁腈、环丁砜、二甲基亚砜、辛醇、丙烯酸
- 主营：颗粒过滤效率检测仪、微生物限度、恒温恒湿箱、培养基、过氧化氢消毒机、超净工作台、生化培养箱、多功能拉力机、生物安全柜、气调保鲜箱、风量仪、电热鼓风干燥箱、总有机碳分析仪、微粒检测仪、口罩死腔测试仪、浮游菌采样器、尘埃粒子计数器、气相色谱仪、口罩密合性检测仪、阻水性能测试仪、呼吸阻力测试仪、洛氏硬度计、干态落絮、口罩下方视野计、针管流量测试仪.
- 主营：氯化亚铁、对甲苯磺酸、十溴二苯乙烷、培养基、硼酸锌、促进剂DMP-30、叔丁基过氧化氢TBH、木材防腐剂ACQ、癸二酸二辛酯DOS、磷酸三乙酯TEP、硫酸锆、有机锡T12、三羟甲基丙烷、K54促进剂、N-甲基吡咯烷酮、雕白粉、衣康酸、酚醛树脂2402、氢氧化铝、溴酸钠、吊白块
- 主营：复合维生素、阿拉伯胶、刺槐豆胶、培养基、瓜尔豆胶 瓜尔胶、果胶、海藻酸钠、黄原胶、结冷胶 低酰基结冷胶、聚丙烯酸钠、卡波姆、卡拉胶、可得然胶、魔芋粉、琼脂 琼脂粉、羧甲基纤维素钠、明胶、温轮胶 威兰胶、聚维酮K30、乙基纤维素、D-氨基葡萄糖盐酸盐、壳聚糖、壳寡糖、酪蛋白 干酪素
- 主营：L-半胱氨酸、L-苯丙氨酸、L-谷氨酸、培养基、L-蛋氨酸、L-谷氨酰胺、L-瓜氨酸、L-精氨酸、L-赖氨酸、L-酪氨酸、L-亮氨酸、L-色氨酸、L-天门冬氨酸、L-缬氨酸、L-异亮氨酸、L-组氨酸、甘氨酸、γ-氨基丁酸、L-谷氨酸钾、维生素A、维生素B1、维生素B12、维生素B2、维生素B5、维生素B6、维生素D3