Overview
The 100 micron cell strainer is a specialized filtration device designed for biological and medical laboratories. It features a mesh with uniform 100-micron pores to separate cells or particles from suspensions. This tool is widely used in cell culture, stem cell research, and tissue engineering to ensure sample homogeneity. Constructed from materials like nylon, stainless steel, or polypropylene, the strainer is compatible with most laboratory protocols. Its design minimizes cell damage during filtration, making it indispensable for applications requiring high viability and purity.
Structure and Working Principle
The strainer consists of a mesh screen mounted in a plastic or metal frame, often with a collection cup. The 100-micron pores allow smaller particles to pass through while retaining larger cells or aggregates. This size-based separation is critical for preparing single-cell suspensions or removing debris. When a cell suspension is poured through the strainer, gravity or gentle pressure forces the liquid through the mesh. The retained cells can then be washed or resuspended. The strainer’s efficiency depends on pore uniformity and material durability.
Key Features
The 100 micron cell strainer offers several advantages, including precision filtration and reusability. Its pore size is tightly controlled to ensure consistent results across batches. The strainer can be sterilized via autoclaving or ethanol immersion, reducing contamination risks. Materials like nylon provide flexibility and chemical resistance, while stainless steel offers robustness for high-throughput workflows. Some models include pre-sterilized, disposable options for convenience. The strainer’s compatibility with standard laboratory equipment enhances its versatility.
Application Areas
This strainer is vital in cell biology, particularly for isolating primary cells, dissociated tissues, or organoids. It is also used in immunology to prepare lymphocyte suspensions and in cancer research to separate tumor cells. Industrial applications include bioprocessing and quality control in pharmaceuticals. In tissue engineering, the strainer helps create uniform cell scaffolds. Its role in downstream assays, such as flow cytometry or PCR, ensures accurate data by removing clumps or contaminants.
Maintenance and Precautions
To maintain performance, rinse the strainer thoroughly after each use to prevent pore clogging. For reusable models, autoclave at 121°C or soak in 70% ethanol. Avoid scrubbing the mesh to preserve pore integrity. Excessive force during filtration can damage cells or deform the mesh. Always check for tears or irregularities before use. Store the strainer in a dry, clean environment to prevent microbial growth or material degradation.
B2B Procurement Guide
When purchasing in bulk, verify the strainer’s material compatibility with your samples and sterilization methods. Compare pore tolerance (±5 microns) to ensure consistency. Reputable suppliers provide certifications for mesh quality and sterility. Consider disposable options for sterile workflows or reusable ones for cost savings. Request samples to test flow rate and cell retention. Bulk pricing typically reduces costs by 10-30%, with discounts for long-term contracts.
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