Overview
The 100μL micropipette is a fundamental tool in laboratories requiring precise liquid measurements. Designed for volumes between 10-100μL, it minimizes human error in repetitive tasks like reagent dispensing or sample aliquoting. Its accuracy (±1-3%) makes it indispensable in life sciences and diagnostics. Modern variants feature adjustable volume settings, digital displays (for electronic models), and lightweight materials to reduce user fatigue. Compliance with ISO 8655 standards ensures reliability across research and industrial applications.
Structure and Working Principle
A 100μL micropipette consists of a plunger, volume adjustment dial, tip ejector, and disposable polypropylene tips. The air displacement mechanism creates a vacuum to aspirate liquid, with accuracy dependent on proper tip sealing and user technique. Internal components include a stainless-steel piston and spring, while the body uses chemically resistant plastics. Electronic models replace manual plungers with motorized controls for enhanced reproducibility in high-throughput workflows.
Key Features
Ergonomics are prioritized with contoured grips and low plunger forces (e.g., 3N) to prevent repetitive strain. Many models offer quick-release tip ejectors and autoclavability (121°C) for sterilization. Advanced features include multi-channel designs (for 96-well plates) and Bluetooth-enabled data logging. Calibration locks and color-coded volume ranges (e.g., yellow for 100μL) streamline workflow organization.
Application Areas
Common in molecular biology (DNA/RNA work), clinical diagnostics (serum pipetting), and pharmaceutical QC. Used for PCR master mixes, cell culture media preparation, and serial dilutions. Industrial applications include food safety testing and environmental monitoring. Multi-channel 100μL pipettes are standard in ELISA and high-throughput screening (HTS) labs.
Maintenance and Precautions
Monthly calibration is recommended using gravimetric methods. Lubricate pistons annually with manufacturer-approved grease. Avoid prolonged exposure to UV or corrosive vapors. Always pre-wet tips for volatile liquids and maintain vertical pipetting angles. Store pipettes upright when not in use. Contamination risks are reduced by using filter tips for sensitive assays.
B2B Procurement Guide
For bulk purchases (10+ units), negotiate service contracts including free calibration and spare parts. Verify supplier certifications (ISO 17025 for calibration labs). Consider total cost of ownership: manual pipettes cost less upfront but electronic models reduce training time. OEM tip compatibility can significantly impact long-term expenses. Request material certificates for regulatory compliance (e.g., FDA 21 CFR Part 11 for electronic models).
Related Manufacturers
- 主营：金属浴、助吸器、移液器、均质机、脱色摇床、微量离心机、电子滴定器、台式离心机、固定混匀仪、滚轴混匀仪、细胞破碎仪、磁力搅拌器、数控加热板、连续分配器、旋转蒸发仪、基因扩增仪、真空吸液器、均质分散机、低速离心机、掌上离心机、电子搅拌器、瓶口分液器、分光光度计、数显加热板、96孔板混匀仪
- 主营：移液器
- 主营：分析仪、防护服、监测仪、压力计、报警仪、粉尘仪、测量仪、水分仪、汞蒸汽、黑度仪、测温仪、测定仪、传感器、速测仪、流量计、采样器、采泥器、风量罩、辐射仪、机器人、测试仪、噪音计、消解仪、防护包、检测箱
- 主营：分析仪、采样器、检测仪、监测仪、照明灯、氟化物、高压气源、气体检测、在线监测、细菌培养、微控制器、监测设备、固定污染源、液液萃取仪、生物安全柜、大气污染物、明渠流量计、红外测油仪、测定重量法、气体报警器、气体探测器、大气采样仪、恒温恒湿系统、双重粉尘过滤、大流量采样泵
- 主营：嵌入皿、冻存管、微量吸头、胎牛血清、离心管、细胞、培养瓶、移液管、离心瓶、培养皿
- 主营：冷凝液、样品瓶、样品瓶盖、滤光片、通用瓶
- 主营：梅思安、多组分、复合气体、环境检测仪、粉尘采样器、气体探测器、甲醛检测仪、烟气分析仪、便携式流速、气体检测仪、红外测温仪、非甲烷总烃、油气回收检测仪
- 主营：10μl、ELISA试剂盒、PCR试剂盒、科研试剂
- 主营：生物安全柜、肺功能检测仪、医用冷藏箱、移液器100、二氧化碳培养箱、经皮黄疸仪、蒸汽灭菌锅、生化分析仪、高压蒸汽灭菌器、医用冰箱、眼科设备、超净工作台、空气消毒机、箱体
- 主营：离心瓶、培养皿、培养瓶、100μl吸头、螺口管、冻存管、离心管、灭菌吸头、滤芯吸头、导电吸头、滤芯吸嘴、工作站吸头、无热源吸头、细胞移液管、细胞培养板、灭菌枪头盒、移液器吸头、三角细胞摇瓶
- 主营：混匀仪、离心机、移液器、干式加热器、数控磁力搅拌器
- 主营：发动机、离合器、机械取力器、hellma、弹簧制动器、液力偶合器、盘式制动器、弹性联轴器、套件泵分动箱、接近控制系统、kpto系列偶合器、动力换挡变速箱
- 主营：D-荧光素钾、D-荧光素钠、缓冲液、100bpDNALadder、裂解液、脲酶Urease、刀豆凝集素ConA、黄嘌呤氧化酶、胰α淀粉酶、β-葡萄糖苷酶、辣根过氧化物酶、生化试剂盒、tRNA转运核糖核酸、胰蛋白酶抑制剂、乙酰胆碱酯酶、L-乳酸脱氢酶、过氧化氢酶、葡萄糖氧化酶、肝素钠、福林酚、三羟甲基氨基甲烷、二硫苏糖醇DTT、苏木色精、伊红Y(曙红)、乙基紫
- 主营：抗体、酶联免疫试剂盒、elisa试剂盒、1000μl、pcr试剂盒、核酸试剂盒
- 主营：水质分析仪器、天平、培养箱